ENPP2 Antibody (Center) | MyBioSource MBS9202571 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ENPP2 Antibody (Center)

catalog :

MBS9202571

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-ENPP2 Antibody (Center) at 1:2000 dilution + Human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 99 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

Western blot analysis of lysates from human placenta and plasma tissue lysate (from left to right), using ENPP2 Antibody (Center). MBS9202571 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

image 3 :

Western blot analysis of (arrow) using rabbit polyclonal ENPP2 Antibody (Center). 293T cell lysates (?ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the gene.

product information

catalog number :

MBS9202571

products type :

Antibody

products full name :

ENPP2 Antibody (Center)

products short name :

[ENPP2]

products name syn :

[Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; Autotaxin; Extracellular lysophospholipase D; LysoPLD; ENPP2; ATX; PDNP2]

other names :

[ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform 2 preproprotein; Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; ectonucleotide pyrophosphatase/phosphodiesterase family member 2; ectonucleotide pyrophosphatase/phosphodiesterase 2; Autotaxin; Extracellular lysophospholipase D; LysoPLD]

products gene name :

[ENPP2]

other gene names :

[ENPP2; ENPP2; ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA; ATX; PDNP2; E-NPP 2; LysoPLD]

uniprot entry name :

ENPP2_HUMAN

clonality :

Polyclonal

isotype :

Rabbit IgG

host :

Rabbit

reactivity :

Human

sequence positions :

[377-406]

sequence length :

888

specificity :

This ENPP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-406 amino acids from the Central region of human ENPP2.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

concentration :

Vial Concentration: 0.37

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)

app notes :

WB~~1:1000. FC~~1:10~50

image1 heading :

Western Blot

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

image4 heading :

Western Blot (WB)

image4 description :

Western blot analysis of ENPP2 (arrow) using rabbit polyclonal ENPP2 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ENPP2 gene (Lane 2) (Origene Technologies).

image5 heading :

Western Blot (WB)

image5 description :

Western blot analysis of ENPP2 Antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). ENPP2 (arrow) was detected using the purified Pab.

other info1 :

Clone Name: RB18117

other info2 :

Antigen Source: HUMAN. Cellular Location: Secreted. Note=Secreted by most body fluids including serum and CSF. Also by adipocytes and numerous cancer cells. Tissue Location: Detected in blood plasma (at protein level)(PubMed:12176993, PubMed:26371182). Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues.

products categories :

Crown Antibodies; Cancer; Cardiovascular; Metabolism; Neuroscience; Signal Transduction

products description :

ENPP2 functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This protein stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The protein is secreted and further processed to make the biologically active form.

products references :

Kawagoe H., Soma O., Goji J., Nishimura N., Narita M.,Genomics 30:380-384(1995). Nam S.W., Clair T., Kim Y.S., McMarlin A.,Cancer Res. 61:6938-6944(2001). The MGC Project Team Genome Res. 14:2121-2127(2004)

ncbi gi num :

91823602

ncbi acc num :

NP_001035181.1

ncbi gb acc num :

NM_001040092.2

uniprot acc num :

Q13822

ncbi mol weight :

98994

ncbi summary :

The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2008]

uniprot summary :

ENPP2: Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Lipid Metabolism - ether lipid; Phosphodiesterase; Secreted; EC 3.1.4.39; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 8q24.1. Cellular Component: extracellular space; integral to plasma membrane; plasma membrane. Molecular Function: phosphodiesterase I activity; nucleotide diphosphatase activity; nucleic acid binding; hydrolase activity; zinc ion binding; calcium ion binding; transcription factor binding; alkylglycerophosphoethanolamine phosphodiesterase activity; scavenger receptor activity; lysophospholipase activity; polysaccharide binding. Biological Process: receptor-mediated endocytosis; G-protein coupled receptor protein signaling pathway; phospholipid catabolic process; immune response; chemotaxis; cell motility; regulation of angiogenesis; phosphate metabolic process; regulation of cell migration

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage