Human Mitochondrial brown fat uncoupling protein 1, UCP1 ELISA Kit | MyBioSource MBS920255 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Mitochondrial brown fat uncoupling protein 1, UCP1 ELISA Kit

catalog :

MBS920255

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS920255

products type :

ELISA Kit

products full name :

Human Mitochondrial brown fat uncoupling protein 1, UCP1 ELISA Kit

products short name :

uncoupling protein 1 (mitochondrial, proton carrier)

products name syn :

Human Mitochondrial brown fat uncoupling protein 1 (UCP1) ELISA kit; SLC25A7; UCP; mitochondrial brown fat uncoupling protein; thermogenin; uncoupling protein 1; uncoupling protein 1 (mitochondrial; proton carrier)

other names :

mitochondrial brown fat uncoupling protein 1; Mitochondrial brown fat uncoupling protein 1; mitochondrial brown fat uncoupling protein 1; thermogenin; solute carrier family 25 member 7; uncoupling protein 1 (mitochondrial, proton carrier); Solute carrier family 25 member 7; Thermogenin

products gene name :

UCP1

other gene names :

UCP1; UCP1; UCP; SLC25A7; SLC25A7; UCP; UCP 1

uniprot entry name :

UCP1_HUMAN

reactivity :

Human

sequence length :

307

specificity :

This assay has high sensitivity and excellent specificity for detection of human UCP1. No significant cross-reactivity or interference between human UCP1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of human UCP1 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for UCP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UCP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UCP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UCP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

11225256

ncbi acc num :

NP_068605.1

ncbi gb acc num :

NM_021833.4

uniprot acc num :

P25874

ncbi mol weight :

33,005 Da

ncbi pathways :

Adipogenesis Pathway (198832); Electron Transport Chain Pathway (198860); Huntington's Disease Pathway (83100); Huntington's Disease Pathway (512); Metabolism Pathway (477135); Mitochondrial Uncoupling Proteins Pathway (160963); PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450); Respiratory Electron Transport, ATP Synthesis By Chemiosmotic Coupling, And Heat Production By Uncoupling Proteins. Pathway (105921); The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway (477137)

ncbi summary :

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq, Jul 2008]

uniprot summary :

UCP1: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Mitochondrial. Chromosomal Location of Human Ortholog: 4q28-q31. Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane. Molecular Function: oxidative phosphorylation uncoupler activity. Biological Process: regulation of transcription from RNA polymerase II promoter; cellular metabolic process; proton transport; brown fat cell differentiation; mitochondrial transport. Disease: Obesity

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage