Human Proprotein convertase subtilisin/kexin type 9, PCSK9 ELISA Kit | MyBioSource MBS920252 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Proprotein convertase subtilisin/kexin type 9, PCSK9 ELISA Kit

catalog :

MBS920252

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

product information

catalog number :

MBS920252

products type :

ELISA Kit

products full name :

Human Proprotein convertase subtilisin/kexin type 9, PCSK9 ELISA Kit

products short name :

[proprotein convertase subtilisin/kexin type 9]

products name syn :

[Human Proprotein convertase subtilisin/kexin type 9 (PCSK9) ELISA kit; FH3; HCHOLA3; LDLCQ1; NARC-1; NARC1; convertase subtilisin/kexin type 9 preproprotein; neural apoptosis regulated convertase 1; proprotein convertase subtilisin/kexin type 9]

other names :

[proprotein convertase subtilisin/kexin type 9 preproprotein; Proprotein convertase subtilisin/kexin type 9; proprotein convertase subtilisin/kexin type 9; subtilisin/kexin-like protease PC9; neural apoptosis regulated convertase 1; convertase subtilisin/kexin type 9 preproprotein; proprotein convertase subtilisin/kexin type 9; Neural apoptosis-regulated convertase 1; NARC-1; Proprotein convertase 9; PC9; Subtilisin/kexin-like protease PC9]

products gene name :

[PCSK9]

other gene names :

[PCSK9; PCSK9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3; NARC1; PSEC0052; NARC-1; PC9]

uniprot entry name :

PCSK9_HUMAN

reactivity :

Human

sequence length :

692

specificity :

This assay has high sensitivity and excellent specificity for detection of human PCSK9. No significant cross-reactivity or interference between human PCSK9 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 0.45 ng/ml -30 ng/ml. Sensitivity: 0.11 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PCSK9. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for PCSK9. The competitive inhibition reaction is launched between with pre-coated PCSK9 and PCSK9 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of PCSK9 in the samples. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

31317307

ncbi acc num :

NP_777596.2

ncbi gb acc num :

NM_174936.3

uniprot acc num :

Q8NBP7

ncbi mol weight :

74,286 Da

ncbi summary :

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

uniprot summary :

PCSK9: Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways. Defects in PCSK9 are the cause of hypercholesterolemia autosomal dominant type 3 (HCHOLA3). A familial condition characterized by elevated circulating cholesterol contained in either low-density lipoproteins alone or also in very-low-density lipoproteins. Belongs to the peptidase S8 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Protease; Cell development/differentiation; EC 3.4.21.-; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p32.3. Cellular Component: Golgi apparatus; extracellular space; cell surface; rough endoplasmic reticulum; lysosome; endoplasmic reticulum; early endosome; ER to Golgi transport vesicle; extrinsic to external side of plasma membrane; perinuclear region of cytoplasm; cytoplasm; late endosome; plasma membrane. Molecular Function: very-low-density lipoprotein binding; sodium channel inhibitor activity; protein binding; protein self-association; low-density lipoprotein receptor binding; serine-type endopeptidase activity; low-density lipoprotein binding; apolipoprotein binding; apolipoprotein receptor binding. Biological Process: cholesterol metabolic process; lysosomal transport; apoptosis; positive regulation of receptor internalization; lipoprotein metabolic process; regulation of low-density lipoprotein receptor catabolic process; proteolysis; cellular response to starvation; liver development; neuron differentiation; protein autoprocessing; cholesterol homeostasis; triacylglycerol metabolic process; cellular response to insulin stimulus; neurogenesis; phospholipid metabolic process; positive regulation of neuron apoptosis; regulation of neuron apoptosis; low-density lipoprotein receptor catabolic process; negative regulation of receptor recycling; regulation of receptor activity; kidney development. Disease: Hypercholesterolemia, Autosomal Dominant, 3

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

435

size3 :

96-Strip-Wells

price3 :

625

size4 :

5x96-Strip-Wells

price4 :

2595

size5 :

10x96-Strip-Wells

price5 :

4905