catalog number :
MBS920230
products type :
ELISA Kit
products full name :
Human Guanine nucleotide-binding protein G, GNAQ ELISA Kit
products short name :
guanine nucleotide binding protein (G protein) , q polypeptide
products name syn :
Human Guanine nucleotide-binding protein G (GNAQ) ELISA kit; RP11-494N1.1; G-ALPHA-q; GAQ; ; guanine nucleotide binding protein (G protein); q polypeptide
other names :
guanine nucleotide-binding protein G(q) subunit alpha; Guanine nucleotide-binding protein G(q) subunit alpha; guanine nucleotide-binding protein G(q) subunit alpha; guanine nucleotide-binding protein G(q) subunit alpha; G alpha q; guanine nucleotide-binding protein alpha-q; Guanine nucleotide-binding protein alpha-q
products gene name :
GNAQ
other gene names :
GNAQ; GNAQ
uniprot entry name :
GNAQ_CANFA
specificity :
This assay has high sensitivity and excellent specificity for detection of human GNAQ. No significant cross-reactivity or interference between human GNAQ and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates and Cell lysates. Assay Type: Sandwich. Detection Range: 39.07 pg/ml -2500 pg/ml. Sensitivity: The minimum detectable dose of human GNAQ is typically less than 9.77 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GNAQ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GNAQ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GNAQ is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GNAQ bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001003249.1
ncbi gb acc num :
NM_001003249.1
ncbi mol weight :
42,142 Da
ncbi pathways :
Adrenergic Signaling In Cardiomyocytes Pathway (908261); Adrenergic Signaling In Cardiomyocytes Pathway (909696); African Trypanosomiasis Pathway (194390); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (84147); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167342); Amoebiasis Pathway (167191); Calcium Regulation In The Cardiac Cell Pathway (198600); Calcium Signaling Pathway (84100)
uniprot summary :
Function: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
size4 :
10x96-Strip-Wells