GNAT1 Antibody (C-term) | MyBioSource MBS9202117 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

GNAT1 Antibody (C-term)

catalog :

MBS9202117

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of GNAT1 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). GNAT1 (arrow) was detected using the purified Pab.

image 2 :

GNAT1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GNAT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

image 3 :

GNAT1 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

product information

catalog number :

MBS9202117

products type :

Antibody

products full name :

GNAT1 Antibody (C-term)

products short name :

[GNAT1]

products name syn :

[Guanine nucleotide-binding protein G(t) subunit alpha-1; Transducin alpha-1 chain; GNAT1; GNATR]

other names :

[guanine nucleotide-binding protein G(t) subunit alpha-1; Guanine nucleotide-binding protein G(t) subunit alpha-1; guanine nucleotide-binding protein G(t) subunit alpha-1; guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1; Transducin alpha-1 chain]

products gene name :

[GNAT1]

other gene names :

[GNAT1; GNAT1; GBT1; GNATR; CSNB1G; CSNBAD3; GNATR]

uniprot entry name :

GNAT1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Mouse, Xenopus)

sequence positions :

[290-318]

sequence length :

350

specificity :

This GNAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-318 amino acids from the C-terminal region of human GNAT1.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

WB, IHC-P, FC, E

app notes :

WB~~1:1000. IHC-P~~1:50~100. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Flow Cytometry (FC/FACS)

other info1 :

Function: Functions as signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina (PubMed: 22190596 ). Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding. Signaling is mediated via downstream effector proteins, such as cGMP-phosphodiesterase (By similarity). Cellular Location: Cell projection, cilium, photoreceptor outer segment {ECO:0000250 UniProtKB:P04695}. Membrane {ECO:0000250 UniProtKB:P04695}; Peripheral membrane protein {ECO:0000250 UniProtKB:P04695}. Tissue Location: Rod photoreceptor cells (PubMed:1614872). Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium (PubMed:22190596)

products categories :

Neuroscience; Signal Transduction

products description :

products references :

Luttrell, L.M. Mol. Biotechnol. 39(3):239-264(2008). Szabo, V., et al. Hum. Mutat. 28(7):741-742(2007). Yi, H.M., et al. Ai Zheng 26(1):9-14(2007). Oldham, W.M., et al. Nat. Struct. Mol. Biol. 13(9):772-777(2006). Muzny, D.M., et al. Nature 440(7088):1194-1198(2006)

ncbi gi num :

22027520

ncbi acc num :

NP_000163.2

ncbi gb acc num :

NM_000172.3

uniprot acc num :

P11488

ncbi pathways :

Amino Sugar And Nucleotide Sugar Metabolism Pathway (2779); Amino Sugar And Nucleotide Sugar Metabolism Pathway (2779); Amino Sugar And Nucleotide Sugar Metabolism Pathway (2779); Amino Sugar And Nucleotide Sugar Metabolism Pathway (2779); Amino Sugar And Nucleotide Sugar Metabolism Pathway (2779); Amino Sugar And Nucleotide Sugar Metabolism Pathway (2779); Amino Sugar And Nucleotide Sugar Metabolism Pathway (2779); Amino Sugar And Nucleotide Sugar Metabolism Pathway (2779); Amino Sugar And Nucleotide Sugar Metabolism Pathway (2779); Amino Sugar And Nucleotide Sugar Metabolism Pathway (2779)

ncbi summary :

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009]

uniprot summary :

G-alpha t1: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. Defects in GNAT1 are the cause of congenital stationary night blindness autosomal dominant type 3 (CSNBAD3); also known as congenital stationary night blindness Nougaret type. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision. Belongs to the G-alpha family. G(i/o/t/z) subfamily. Protein type: G protein, heterotrimeric; G protein; G protein, heterotrimeric alpha G((i/o/t/z)). Chromosomal Location of Human Ortholog: 3p21. Cellular Component: photoreceptor outer segment; photoreceptor inner segment; membrane; cell soma; apical plasma membrane; plasma membrane; heterotrimeric G-protein complex; cytosol; photoreceptor connecting cilium. Molecular Function: GTPase activity; signal transducer activity; GDP binding; acyl binding; GTP binding; G-protein-coupled receptor binding; metal ion binding; G-protein beta/gamma-subunit binding; protein kinase binding. Biological Process: response to light stimulus; phototransduction, visible light; sensory perception of umami taste; metabolic process; response to light intensity; detection of chemical stimulus involved in sensory perception of bitter taste; signal transduction; rhodopsin mediated signaling; G-protein signaling, coupled to cAMP nucleotide second messenger; cell proliferation; eye photoreceptor cell development; regulation of rhodopsin mediated signaling; visual perception; retina development in camera-type eye; detection of light stimulus involved in visual perception; positive regulation of cyclic-nucleotide phosphodiesterase activity; negative regulation of cyclic-nucleotide phosphodiesterase activity. Disease: Night Blindness, Congenital Stationary, Type 1g; Night Blindness, Congenital Stationary, Autosomal Dominant 3

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage