Natriuretic Peptide Receptor C Antibody (N-term) | MyBioSource MBS9201988 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Natriuretic Peptide Receptor C Antibody (N-term)

catalog :

MBS9201988

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-ANPC (S82) at 1:1000dilution Lane 1: Hela whole cell lysate Lane 2:HL-60 whole cell lysate Lysates/proteins at 20?g per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kD Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

Fluorescent confocal image of HeLa cells stained with Natriuretic Peptide Receptor C(N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP8113a Natriuretic PeptideReceptor C (N-term) primary antibody (1:200, 2h at room temperature). For secondary antibody, 488 conjugated donkeyanti-rabbit antibody (green) was used (1:1000,1h). Nuclei were counterstained with Hoechst33342 (blue) (10 ?g/ml, 5 min). Note the highly specific localization of the Natriuretic PeptideReceptor C mainly to the mainly to the nucleus.

image 3 :

Natriuretic Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody (N-term) (Cat#MBS9201988)immunohistochemistry analysis informalin fixed and paraffin embedded human kidney tissue followed bY peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Natriuretic Peptide Receptor C (NPR3/ANPC)Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

product information

catalog number :

MBS9201988

products type :

Antibody

products full name :

Natriuretic Peptide Receptor C Antibody (N-term)

products short name :

[Natriuretic Peptide Receptor C]

products name syn :

[Atrial natriuretic peptide receptor 3; Atrial natriuretic peptide clearance receptor; Atrial natriuretic peptide receptor type C; ANP-C; ANPR-C; NPR-C; NPR3; ANPRC; C5orf23; NPRC]

other names :

[atrial natriuretic peptide receptor 3 isoform 2; Atrial natriuretic peptide receptor 3; atrial natriuretic peptide receptor 3; natriuretic peptide receptor 3; Atrial natriuretic peptide clearance receptor; Atrial natriuretic peptide receptor type C; ANP-C; ANPR-C; NPR-C]

products gene name :

[NPR3]

other gene names :

[NPR3; NPR3; NPRC; ANP-C; ANPRC; NPR-C; ANPR-C; GUCY2B; C5orf23; ANPRC; C5orf23; NPRC; ANP-C; ANPR-C; NPR-C]

uniprot entry name :

ANPRC_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence length :

540

specificity :

This Natriuretic Peptide Receptor C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-97 amino acids from the N-terminal region of human Natriuretic Peptide Receptor C.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

WB, IF, IHC-P, E

app notes :

WB~~1:1000. IF~~1:200. IHC-P~~1:10~50

image1 heading :

All lanes Anti-ANPC

image2 heading :

Fluorescent

image3 heading :

Natriuretic NT

other info1 :

. Cellular location: Membrane; Single-pass type I membrane protein. Antigen Region: 67-97. Antigen Type: Synthetic Peptide

other info2 :

Antigen Source: HUMAN

products categories :

Cardiovascular; Signal Transduction

products description :

ANPC is a receptor for atrial natriuretic peptide. It does not exhibit guanylate cyclase activity. There seem to be at least three ANP receptors: two with guanylate cyclase activity (ANPA and ANPB) and one (ANPC) which is probably responsible for the clearance of ANP from the circulation without a role in signal transduction.

products references :

References for protein:. 1.Porter, J.G., et al., Biochem. Biophys. Res. Commun. 171(2):796-803 (1990). 2.Lowe, D.G., et al., Nucleic Acids Res. 18 (11), 3412 (1990). References for HeLa cell line:. 1. Scherer WF, Syverton JT, Gey GO (May 1953). "Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix". J. Exp. Med. 97 (5): 695-710. [PubMed:13052828]. 2. Macville M, Schröck E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T (January 1999). "Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping". Cancer Res. 59 (1): 141-50. [PubMed: 9892199]. 3. Rahbari R, Sheahan T, Modes V, Collier P, Macfarlane C, Badge RM (April 2009). "A novel L1 retrotransposon marker for HeLa cell line identification". BioTechniques 46 (4): 277-84. [PubMed: 19450234]. 4. Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (July 2010). "Check your cultures! A list of cross-contaminated or misidentified cell lines". Int. J. Cancer 127 (1): 1-8. [PubMed:20143388].

ncbi gi num :

4505441

ncbi acc num :

NP_000899.1

ncbi gb acc num :

NM_000908.3

uniprot acc num :

P17342

ncbi summary :

This gene encodes one of three natriuretic peptide receptors. Natriutetic peptides are small peptides which regulate blood volume and pressure, pulmonary hypertension, and cardiac function as well as some metabolic and growth processes. The product of this gene encodes a natriuretic peptide receptor responsible for clearing circulating and extracellular natriuretic peptides through endocytosis of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

uniprot summary :

ANP-C: Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity. Belongs to the ANF receptor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, GPCR. Chromosomal Location of Human Ortholog: 5p13.3. Cellular Component: integral to plasma membrane. Molecular Function: protein homodimerization activity; hormone binding; natriuretic peptide receptor activity; peptide hormone binding; peptide receptor activity, G-protein coupled. Biological Process: regulation of osteoblast proliferation; phosphoinositide-mediated signaling; negative regulation of smooth muscle cell proliferation; positive regulation of nitric-oxide synthase activity; regulation of blood pressure; negative regulation of adenylate cyclase activity; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); G-protein signaling, adenylate cyclase inhibiting pathway; pancreatic juice secretion; skeletal development

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage