Activin A Receptor Type IB (ACVR1B) Antibody (N-term) | MyBioSource MBS9201918 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Activin A Receptor Type IB (ACVR1B) Antibody (N-term)

catalog :

MBS9201918

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

All lanes : Anti-Activin A Receptor Type IB(ACVR1B) Antibody (N-term) at 1:1000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lane 3: Rat brain lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

All lanes : Anti-Activin A Receptor Type IB(ACVR1B) Antibody (N-term) at 1:1000 dilution Lane 1: Human brain lysate Lane 2: Human liver lysate Lane 3: Mouse brain lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image 3 :

Anti-Activin A Receptor Type IB (ACVR1B) Antibody (N-term) at 1:1000 dilution + Human liver lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9201918

products type :

Antibody

products full name :

Activin A Receptor Type IB (ACVR1B) Antibody (N-term)

products short name :

[Activin A Receptor Type IB (ACVR1B)]

products name syn :

[Activin receptor type-1B; Activin receptor type IB; ACTR-IB; Activin receptor-like kinase 4; ALK-4; Serine/threonine-protein kinase receptor R2; SKR2; ACVR1B; ACVRLK4; ALK4]

other names :

[activin receptor type-1B isoform a; Activin receptor type-1B; activin receptor type-1B; activin A receptor, type IB; Activin receptor type IB; ACTR-IB; Activin receptor-like kinase 4; ALK-4; Serine/threonine-protein kinase receptor R2; SKR2]

products gene name :

[ACVR1B]

other gene names :

[ACVR1B; ACVR1B; ALK4; SKR2; ACTRIB; ACVRLK4; ACVRLK4; ALK4; ACTR-IB; ALK-4; SKR2]

uniprot entry name :

ACV1B_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, Mouse (Predicted Reactivity: Rat)

sequence positions :

[39-68]

sequence length :

505

specificity :

This Activin A Receptor Type IB (ACVR1B) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the N-terminal region of human Activin A Receptor Type IB (ACVR1B).

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)

app notes :

WB~~1:1000. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

image4 heading :

Western Blot (WB)

image4 description :

The anti-ACVR1B Pab is used in Western blot to detect ACVR1B in mouse kidney tissue lysate.

image5 heading :

Western Blot (WB)

image5 description :

ACVR1B Antibody (N-term)western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ACVR1B antibody detected the ACVR1B protein (arrow).

other info1 :

Function: Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine- threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C- terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2.

other info2 :

Cellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Location: Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver

products categories :

Signal Transduction

products description :

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with a cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling, and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. The gene for ACVR1B (activin A type IB receptor) is composed of 11 exons. Alternative splicing and alternative polyadenylation result in 3 fully described transcript variants. The mRNA expression of variants 1, 2, and 3 is confirmed, and a potential fourth variant contains an alternative exon 8 and lacks exons 9 through 11, but its mRNA expression has not been confirmed.

products references :

Harrison, C.A., et al., J. Biol. Chem. 278(23):21129-21135 (2003). Mukasa, C., et al., Endocrinology 144(4):1603-1611 (2003). Danila, D.C., et al., J. Clin. Endocrinol. Metab. 87(10):4741-4746 (2002). Schneider-Kolsky, M.E., et al., Placenta 23(4):294-302 (2002). Roijer, E., et al., Mamm. Genome 9(3):266-268 (1998).

ncbi gi num :

4757720

ncbi acc num :

NP_004293.1

ncbi gb acc num :

NM_004302.4

uniprot acc num :

P36896

ncbi mol weight :

56807

ncbi pathways :

Folate Polyglutamylation Pathway (91); Folate Polyglutamylation Pathway (91); Folate Polyglutamylation Pathway (91); Folate Polyglutamylation Pathway (91); Folate Polyglutamylation Pathway (91); Folate Polyglutamylation Pathway (91); Folate Polyglutamylation Pathway (91); Folate Polyglutamylation Pathway (91); Folate Polyglutamylation Pathway (91); Folate Polyglutamylation Pathway (91)

ncbi summary :

This gene encodes an activin A type IB receptor. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I and two type II receptors. This protein is a type I receptor which is essential for signaling. Mutations in this gene are associated with pituitary tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]

uniprot summary :

ALK4: Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine- threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C- terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2. ACVRIB is abundantly expressed in systemic sclerosis patient fibroblasts and production of collagen is also induced by activin-A/INHBA. This suggests that the activin/ACRV1B signaling mechanism is involved in systemic sclerosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.7.11.30; Protein kinase, Ser/Thr (receptor); Membrane protein, integral; Protein kinase, TKL; Kinase, protein; TKL group; STKR family; Type1 subfamily. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: cell surface; integral to plasma membrane; plasma membrane; activin receptor complex; receptor complex. Molecular Function: activin receptor activity; metal ion binding; transmembrane receptor protein serine/threonine kinase activity; transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; activin receptor activity, type I; ubiquitin protein ligase binding; growth factor binding; activin binding; SMAD binding; ATP binding; receptor signaling protein serine/threonine kinase activity. Biological Process: development of primary female sexual characteristics; central nervous system development; in utero embryonic development; protein amino acid autophosphorylation; positive regulation of erythrocyte differentiation; activin receptor signaling pathway; peptidyl-threonine phosphorylation; signal transduction; protein amino acid phosphorylation; positive regulation of activin receptor signaling pathway; regulation of transcription, DNA-dependent; hair follicle development; transmembrane receptor protein serine/threonine kinase signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; G1/S transition of mitotic cell cycle

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage