MMP14 Antibody (N-term) | MyBioSource MBS9201843 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MMP14 Antibody (N-term)

catalog :

MBS9201843

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

MBS9201843 staining MMP14 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

image 2 :

Flow cytometric analysis of MCF-7 cells using MMP14 Antibody (N-term) (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

image 3 :

All lanes : Anti-MMP14 Antibody (R160) at 1:2000 dilution:. Lane 1: A2058 whole cell lysate . Lane 2: HT-1080 whole cell lysate . Lane 3: mouse lung lysate . Lane 4: MCF-7 whole cell lysate . Lane 5: NCI-H292 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa . Blocking/Dilution buffer: 5% NFDM/TBST

product information

catalog number :

MBS9201843

products type :

Antibody

products full name :

MMP14 Antibody (N-term)

products short name :

[MMP14]

products name syn :

[Matrix metalloproteinase-14; MMP-14; MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP; MMP14]

other names :

[matrix metalloproteinase-14 preproprotein; Matrix metalloproteinase-14; matrix metalloproteinase-14; matrix metallopeptidase 14 (membrane-inserted); MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP]

products gene name :

[MMP14]

other gene names :

[MMP14; MMP14; MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1; MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP]

uniprot entry name :

MMP14_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, Mouse. Predicted: Bovine, Pig, Rat

sequence positions :

[145-174]

sequence length :

582

specificity :

This MMP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the N-terminal region of human MMP14.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBSwith 0.09% (W/V) sodium azide. This antibodyis purified through a protein A column,followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

IHC-P, FC, WB, E

app notes :

IHC-P~~1:25. FC~~1:10~50. WB~~1:1000

image1 heading :

Immunohistochemistry

image2 heading :

Flow cytometric

image3 heading :

Western Blot

image4 heading :

Western Blot

image4 description :

MMP14 Antibody (R160) (Cat.# MBS9201843) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MMP14 antibody detected the MMP14 protein (arrow).

other info2 :

Antigen Source: HUMAN. Function: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro- MMP2. Cellular Location: Membrane; Single-pass type I membrane protein. Melanosome. Cytoplasm Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm. Tissue Location: Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.

products categories :

Crown Antibodies; Cancer; Cardiovascular; Cell Biology; Neuroscience; Signal Transduction

products description :

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP14 seems to specifically activate progelatinase A, and may thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. Expression is significant in stromal cells of colon, breast, and head and neck.

products references :

Will, H., et al., Eur. J. Biochem. 231(3):602-608 (1995). Takino, T., et al., Gene 155(2):293-298 (1995). Okada, A., et al., Proc. Natl. Acad. Sci. U.S.A. 92(7):2730-2734 (1995). Sato, H., et al., Nature 370(6484):61-65 (1994).

ncbi gi num :

4826834

ncbi acc num :

NP_004986.1

ncbi gb acc num :

NM_004995.3

uniprot acc num :

P50281

ncbi pathways :

Citrate Cycle (TCA Cycle) Pathway (4323); Citrate Cycle (TCA Cycle) Pathway (4323); Citrate Cycle (TCA Cycle) Pathway (4323); Citrate Cycle (TCA Cycle) Pathway (4323); Citrate Cycle (TCA Cycle) Pathway (4323); Citrate Cycle (TCA Cycle) Pathway (4323); Citrate Cycle (TCA Cycle) Pathway (4323); Citrate Cycle (TCA Cycle) Pathway (4323); Citrate Cycle (TCA Cycle) Pathway (4323); Citrate Cycle (TCA Cycle) Pathway (4323)

ncbi summary :

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]

uniprot summary :

MMP14: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Up-regulated by NANOS1. Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. Belongs to the peptidase M10A family. Protein type: Membrane protein, integral; Protease; Motility/polarity/chemotaxis; EC 3.4.24.80. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: extracellular matrix; focal adhesion; integral to plasma membrane; Golgi lumen; cytoplasm; melanosome; plasma membrane. Molecular Function: integrin binding; protein binding; protease activator activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding; transcription factor activity. Biological Process: extracellular matrix organization and biogenesis; tissue remodeling; negative regulation of focal adhesion formation; male gonad development; embryonic cranial skeleton morphogenesis; proteolysis; positive regulation of cell growth; extracellular matrix disassembly; collagen catabolic process; branching morphogenesis of a tube; regulation of transcription, DNA-dependent; ovarian follicle development; response to estrogen stimulus; response to mechanical stimulus; endothelial cell proliferation; response to hypoxia; zymogen activation; angiogenesis; response to oxidative stress; astrocyte cell migration; endochondral ossification; positive regulation of cell migration; lung development. Disease: Winchester Syndrome

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage