Phospho-CDC25A (S124) Antibody | MyBioSource MBS9201824 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Phospho-CDC25A (S124) Antibody

catalog :

MBS9201824

quantity :

0.08 mL

price :

150 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

The anti-phospho CDC25A S124 Pab is used in Western blot to detect CDC25A in mouse liver tissue lysate

image 2 :

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

image 3 :

Western blot analysis of lysates from 293 cell line, untreated or treated with Alkaline phosphatase, 1h, using 459088102 (upper) or Beta-actin (lower).

product information

catalog number :

MBS9201824

products type :

Antibody

products full name :

Phospho-CDC25A (S124) Antibody

products short name :

[Phospho-CDC25A (S124)]

products name syn :

[M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A; CDC25A]

other names :

[M-phase inducer phosphatase 1 isoform a; M-phase inducer phosphatase 1; M-phase inducer phosphatase 1; cell division cycle 25A; Dual specificity phosphatase Cdc25A]

products gene name :

[CDC25A]

other gene names :

[CDC25A; CDC25A; CDC25A2]

uniprot entry name :

MPIP1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Rat)

sequence length :

524

specificity :

This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S124 of human CDC25A.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC) - Paraffin

app notes :

WB~~1:1000. IHC-P~~1:50~100

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Western Blot (WB)

image4 heading :

Western Blot (WB)

image4 description :

Western blot analysis of lysates from A2780 cell line, untreated or treated with Alkaline phosphatase, 1h, using 459088101(upper) or Beta-actin (lower).

image5 heading :

Western Blot (WB)

image5 description :

Western blot analysis of lysates from A2780 cell line, untreated or treated with Alkaline phosphatase, 1h, using 459085101 or Beta-actin (lower).

other info1 :

Function: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

products categories :

Cancer; Cell Biology; Phospho Antibodies

products description :

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

products references :

Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004). Nemoto, K., et al., Prostate 58(1):95-102 (2004). Goloudina, A., et al., Cell Cycle 2(5):473-478 (2003). Chen, M.S., et al., Mol. Cell. Biol. 23(21):7488-7497 (2003). Chow, J.P., et al., Mol. Biol. Cell 14(10):3989-4002 (2003).

ncbi gi num :

42490758

ncbi acc num :

NP_001780.2

ncbi gb acc num :

NM_001789.2

uniprot acc num :

P30304

ncbi pathways :

Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993)

ncbi summary :

uniprot summary :

CDC25A: a member of the MPI phosphatase family. Functions as a dosage-dependent inducer in mitotic control. Required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two splice variant isoforms have been described. Protein type: Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis; EC 3.1.3.48. Chromosomal Location of Human Ortholog: 3p21. Cellular Component: nucleoplasm; cytosol. Molecular Function: protein binding; protein tyrosine phosphatase activity; protein kinase binding. Biological Process: mitosis; cell proliferation; response to radiation; regulation of cell cycle; cell division; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; DNA replication; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle

size1 :

0.08 mL

price1 :

150 USD

more info or order :

MyBioSource product webpage