WHSC1 Antibody (C-term) | MyBioSource MBS9201595 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

WHSC1 Antibody (C-term)

catalog :

MBS9201595

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

All lanes : Anti-WHSC1 Antibody (C-term) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 152 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

WHSC1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the WHSC1 antibody detected the WHSC1 protein (arrow).

product information

catalog number :

MBS9201595

products type :

Antibody

products full name :

WHSC1 Antibody (C-term)

products short name :

[WHSC1]

products name syn :

[Histone-lysine N-methyltransferase NSD2; Multiple myeloma SET domain-containing protein; MMSET; Nuclear SET domain-containing protein 2; NSD2; Protein trithorax-5; Wolf-Hirschhorn syndrome candidate 1 protein; WHSC1; WHSC1; KIAA1090; MMSET; NSD2; TRX5]

other names :

[histone-lysine N-methyltransferase NSD2 isoform 1; Histone-lysine N-methyltransferase NSD2; histone-lysine N-methyltransferase NSD2; Wolf-Hirschhorn syndrome candidate 1; Multiple myeloma SET domain-containing protein; MMSET; Nuclear SET domain-containing protein 2; NSD2; Protein trithorax-5; Wolf-Hirschhorn syndrome candidate 1 protein; WHSC1]

products gene name :

[WHSC1]

other gene names :

[WHSC1; WHSC1; WHS; NSD2; TRX5; MMSET; REIIBP; KIAA1090; MMSET; NSD2; TRX5; MMSET; NSD2; WHSC1]

uniprot entry name :

NSD2_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Mouse)

sequence positions :

[1061-1089]

sequence length :

1365

specificity :

This WHSC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1061-1089 amino acids from the C-terminal region of human WHSC1.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

concentration :

Vial Concentration: 0.5

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB~~1:1000

image1 heading :

Western Blot

image2 heading :

Western Blot (WB)

other info1 :

Antigen Type: Synthetic Peptide. Clone Names: RB36971. Function: Histone methyltransferase with histone H3 'Lys-27' (H3K27me) methyltransferase activity. Isoform 2 may act as a transcription regulator that binds DNA and suppresses IL5 transcription through HDAC recruitment. Cellular Location: Nucleus {ECO:0000255 PROSITEProRule: PRU00267, ECO:0000269 PubMed:15677557,ECO:0000269 PubMed:16197452}. Chromosome. Tissue Location: Widely expressed.

other info2 :

Antigen Source: HUMAN

products description :

This gene encodes a protein that contains four domains. present in other developmental proteins: a PWWP domain, an HMG box,. a SET domain, and a PHD-type zinc finger. It is expressed. ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS). is a malformation syndrome associated with a hemizygous deletion of. the distal short arm of chromosome 4. This gene maps to the 165 kb. WHS critical region and has also been involved in the chromosomal. translocation t(4;14)(p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript. variants encoding different isoforms. Some transcript variants are. nonsense-mediated mRNA (NMD) decay candidates, hence not. represented as reference sequences.

products references :

Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :. Kang, H.B., et al. FEBS Lett. 583(12):1880-1886(2009). Kassambara, A., et al. Biochem. Biophys. Res. Commun. 379(4):840-845(2009). Brito, J.L., et al. Haematologica 94(1):78-86(2009). Li, J., et al. Neuro-oncology 10(1):45-51(2008)

ncbi gi num :

109633019

ncbi acc num :

NP_001035889.1

ncbi gb acc num :

NM_001042424.2

uniprot acc num :

O96028

ncbi mol weight :

152258

ncbi summary :

This gene encodes a protein that contains four domains present in other developmental proteins: a PWWP domain, an HMG box, a SET domain, and a PHD-type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and has also been involved in the chromosomal translocation t(4;14)(p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Some transcript variants are nonsense-mediated mRNA (NMD) decay candidates, hence not represented as reference sequences. [provided by RefSeq, Jul 2008]

uniprot summary :

WHSC1: a SET domain-containing protein lysine methyltransferase that is expressed normally in early development. Catalyzes the dimethylation of H3K36; H3K36me2 is associated with regions that are transcriptionally active. Maps in the Wolf-Hirschhorn syndrome critical region and is fused to IgH in about 15% of t(4;14) multiple myeloma cases. Patients with the t(4;14) chromosomal translocation comprise one of the worst prognostic subgroups of multiple myeloma. The t(4;14) translocation results in overexpression of WHSC1 and of FGFR3 due to the placement of the strong immunoglobulin H intronic Emu enhancer and 3-prime enhancer in the promoter regions of WHSC1 and FGFR3 genes, respectively. 100% of t(4;14) patients retain overexpression of WHSC1, while 30% have lost expression of FGFR3, suggesting that WHSC1 and not FGFR3 may the primary driver of the disease. Seven alternatively spliced isoforms of the human protein have been reported. Protein type: Oncoprotein; Methyltransferase; Amino Acid Metabolism - lysine degradation; Ubiquitin conjugating system; Methyltransferase, protein lysine; EC 2.1.1.43. Chromosomal Location of Human Ortholog: 4p16.3. Cellular Component: nucleoplasm; cytoplasm; chromosome; nucleus. Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; chromatin binding; histone-lysine N-methyltransferase activity. Biological Process: establishment and/or maintenance of chromatin architecture; anatomical structure morphogenesis; transcription, DNA-dependent; histone H3-K36 methylation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of isotype switching to IgA isotypes. Disease: Wolf-hirschhorn Syndrome

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage