EDNRB Antibody (C-term) | MyBioSource MBS9201556 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

EDNRB Antibody (C-term)

catalog :

MBS9201556

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of EDNRB Antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane). EDNRB (arrow) was detected using the purified Pab.

image 2 :

EDNRB Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EDNRB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

image 3 :

Confocal immunofluorescent analysis of EDNRB Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

product information

catalog number :

MBS9201556

products type :

Antibody

products full name :

EDNRB Antibody (C-term)

products short name :

[EDNRB]

products name syn :

[ETRB]

other names :

[endothelin B receptor isoform 1; Endothelin B receptor; endothelin B receptor; endothelin receptor type B; Endothelin receptor non-selective type]

products gene name :

[EDNRB]

other gene names :

[EDNRB; EDNRB; ETB; ET-B; ETB1; ETBR; ETRB; HSCR; WS4A; ABCDS; ET-BR; HSCR2; ETRB; ET-B; ET-BR]

uniprot entry name :

EDNRB_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse (Predicted Reactivity: Bovine, Pig, Rabbit, Rat)

sequence positions :

[409-439]

sequence length :

439

specificity :

This EDNRB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 409-439 amino acids from the C-terminal region of human EDNRB.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

app notes :

WB~~1:1000. IHC-P~~1:10~50. IF~~1:10~50. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

image4 heading :

Flow Cytometry (FC/FACS)

image4 description :

EDNRB Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

other info1 :

Antigen Type: Synthetic Peptide. Primary Accession: P24530. Other Accession: P21451, Q9N0W7, P35463, P48302, P28088, O62709

other info2 :

Antigen Source: HUMAN. Function: Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Cellular Location: Cell membrane; Multi-pass membrane protein. Tissue Location : Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells. Precautions: EDNRB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

products categories :

Signal Transduction; GPCR Antibodies

products description :

EDNRB is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene.

products references :

MacClellan,L.R., et.al., Stroke 40 (10), E550-E557 (2009)

ncbi gi num :

4557547

ncbi acc num :

NP_000106.1

ncbi gb acc num :

NM_000115.3

uniprot acc num :

P24530

ncbi mol weight :

49644

ncbi summary :

The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

uniprot summary :

ETB: a G protein-coupled receptor which activates a phosphatidylinoitol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutation in endothelin receptor type B gene. Essential component in the normal development of two neuronal crest-derived cell lineages. Two splice variant isoforms have been described. Protein type: Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 13q22. Cellular Component: nuclear membrane; integral to plasma membrane; plasma membrane; lipid raft. Molecular Function: endothelin receptor activity; protein binding; peptide hormone binding; type 1 angiotensin receptor binding. Biological Process: epithelial fluid transport; cGMP-mediated signaling; regulation of fever; response to pain; negative regulation of cellular protein metabolic process; negative regulation of transcription from RNA polymerase II promoter; vein smooth muscle contraction; sensory perception of pain; vasodilation; response to organic cyclic substance; enteric nervous system development; regulation of epithelial cell proliferation; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; regulation of blood pressure; positive regulation of cell proliferation; melanocyte differentiation; regulation of pH; neural crest cell migration; aging; nervous system development; posterior midgut development; vasoconstriction; negative regulation of adenylate cyclase activity; macrophage chemotaxis; peripheral nervous system development; negative regulation of neuron maturation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of sensory perception of pain; positive regulation of protein amino acid phosphorylation; negative regulation of apoptosis. Disease: Abcd Syndrome; Waardenburg Syndrome, Type 4a; Hirschsprung Disease, Susceptibility To, 2

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage