TSG101 Antibody (N-term) | MyBioSource MBS9201535 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

TSG101 Antibody (N-term)

catalog :

MBS9201535

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

The anti-TSG101 Pab is used in Western blot to detect TSG101 in mouse kidney tissue lysate.

image 2 :

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

product information

catalog number :

MBS9201535

products type :

Antibody

products full name :

TSG101 Antibody (N-term)

products short name :

[TSG101]

products name syn :

[Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101; TSG101]

other names :

[tumor susceptibility gene 101 protein; Tumor susceptibility gene 101 protein; tumor susceptibility gene 101 protein; tumor susceptibility 101; ESCRT-I complex subunit TSG101]

products gene name :

[TSG101]

other gene names :

[TSG101; TSG101; TSG10; VPS23]

uniprot entry name :

TS101_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse

sequence positions :

[1-30]

sequence length :

390

specificity :

This TSG101 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TSG101.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 6 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin(IHC)

app notes :

WB~~1:1000. IHC-P~~1:50~100

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Function: Component of the ESCRT-I complex, aregulator of vesicular trafficking process.Binds to ubiquitinated cargo proteins and isrequired for the sorting of endocyticubiquitinated cargos into multivesicularbodies (MVBs). Mediates the associationbetween the ESCRT-0 and ESCRT-I complex.Required for completion of cytokinesis; thefunction requires CEP55. May be involved incell growth and differentiation. Acts as anegative growth regulator. Involved in thebudding of many viruses through aninteraction with viral proteins that contain alate-budding motif P-[ST]-A-P. This interactionis essential for viral particle budding ofnumerous retroviruses. Required for theexosomal release of SDCBP, CD63 andsyndecan (PubMed: 22660413 ). It may alsoplay a role in the extracellular release ofmicrovesicles that differ from the exosomes. Cellular Location: Cytoplasm. Early endosome membrane;Peripheral membrane protein; Cytoplasmicside. Late endosome membrane; Peripheralmembrane protein. Cytoplasm, cytoskeleton,microtubule organizing center, centrosomeMidbody, Midbody ring. Nucleus. Note=Mainlycytoplasmic. Membrane- associated whenactive and soluble when inactive. Nuclearlocalization is cell cycle-dependent.Interaction with CEP55 is required forlocalization to the midbody during cytokinesis. Tissue Location: Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.

products categories :

Cancer; Cell Biology; Microbiology

products description :

TSG101 belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.

products references :

Favre, M., et al., J. Acquir. Immune Defic. Syndr. 34(2):127-133 (2003). Lu, Q., et al., Proc. Natl. Acad. Sci. U.S.A. 100(13):7626-7631 (2003). Goila-Gaur, R., et al., J. Virol. 77(11):6507-6519 (2003). Blanco, S., et al., FEMS Microbiol. Lett. 221(2):151-154 (2003). Martin-Serrano, J., et al., J. Virol. 77(8):4794-4804 (2003).

ncbi gi num :

5454140

ncbi acc num :

NP_006283.1

ncbi gb acc num :

NM_006292.3

uniprot acc num :

Q99816

ncbi pathways :

Bisphenol Degradation Pathway (7251); Bisphenol Degradation Pathway (7251); Bisphenol Degradation Pathway (7251); Bisphenol Degradation Pathway (7251); Bisphenol Degradation Pathway (7251); Bisphenol Degradation Pathway (7251); Bisphenol Degradation Pathway (7251); Bisphenol Degradation Pathway (7251); Bisphenol Degradation Pathway (7251); Bisphenol Degradation Pathway (7251)

ncbi summary :

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]

uniprot summary :

TSG101: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell cycle regulation; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 11p15. Cellular Component: multivesicular body; late endosome membrane; late endosome; cytoplasm; early endosome; plasma membrane; nucleolus; endosome membrane. Molecular Function: protein binding; ubiquitin binding; protein homodimerization activity; DNA binding; ubiquitin protein ligase binding; transcription corepressor activity; calcium-dependent protein binding. Biological Process: viral reproduction; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; non-lytic virus budding; protein modification process; viral infectious cycle; virus assembly; endosome transport; keratinocyte differentiation; negative regulation of cell proliferation; protein transport; cell division; viral protein processing; regulation of cell growth; cell cycle arrest; negative regulation of transcription, DNA-dependent. Disease: Breast Cancer

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage