ELISA/assay

Mouse NACHT, LRR and PYD domains-containing protein 3, NLRP3 ELISA Kit

MBS920134

48-Strip-Wells

510 USD

ELISA Kit

Mouse NACHT, LRR and PYD domains-containing protein 3, NLRP3 ELISA Kit

NLR family, pyrin domain containing 3

Mouse NACHT; LRR and PYD domains-containing protein 3 (NLRP3) ELISA kit; AGTAVPRL; AII; AII/AVP; AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCU; FLJ95925; MWS; NALP3; PYPAF1; AII/AVP receptor-like; NACHT domain-; leucine-rich repeat-; and PYD-containing protein 3; NACHT; LRR and PY; NLR family; pyrin domain containing 3

NACHT, LRR and PYD domains-containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NACHT, LRR and PYD domains-containing protein 3; cryopyrin; PYRIN-containing APAF1-like protein 1; cold autoinflammatory syndrome 1 homolog; mast cell maturation inducible protein 1; NACHT/LRR/pyrin domain-containing protein 3; cold autoinflammatory syndrome 1 protein homolog; mast cell maturation-associated-inducible protein 1; NLR family, pyrin domain containing 3; Cold autoinflammatory syndrome 1 protein homolog; Cryopyrin; Mast cell maturation-associated-inducible protein 1; PYRIN-containing APAF1-like protein 1

NLRP3

Nlrp3; Nlrp3; FCU; MWS; FCAS; Cias1; Mmig1; NALP3; Pypaf1; AII/AVP; AGTAVPRL; Cias1; Mmig1; Nalp3; Pypaf1

NALP3_MOUSE

Mouse

1033

This assay has high sensitivity and excellent specificity for detection of mouse NLRP3. No significant cross-reactivity or interference between mouse NLRP3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml -400 pg/ml. Sensitivity: The minimum detectable dose of mouse NLRP3 is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NLRP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NLRP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NLRP3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NLRP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

22003870

NP_665826.1

NM_145827.3

Q8R4B8

118,275 Da

Immune System Pathway (971216); Inflammasomes Pathway (1001249); Influenza A Pathway (217174); Influenza A Pathway (217150); Innate Immune System Pathway (971212); NOD-like Receptor Signaling Pathway (122195); NOD-like Receptor Signaling Pathway (122131); Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways (1001247); Pertussis Pathway (218112); Pertussis Pathway (218099)

NLRP3: May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1); also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS); also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA); also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. Belongs to the NLRP family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Inhibitor. Cellular Component: cell; cytoplasm. Molecular Function: protein binding; nucleotide binding; ATP binding. Biological Process: caspase activation; apoptosis; negative regulation of acute inflammatory response; positive regulation of caspase activity; interleukin-1 beta production; positive regulation of interleukin-1 beta secretion; negative regulation of NF-kappaB import into nucleus; activation of NF-kappaB transcription factor; negative regulation of interleukin-1 beta secretion; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; regulation of inflammatory response; interleukin-18 production; interleukin-1 secretion; inflammatory response; defense response to virus

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800