ELISA/assay

Pig Interleukin-23 subunit alpha, IL23A ELISA Kit

MBS920133

48-Strip-Wells

565 USD

ELISA Kit

Pig Interleukin-23 subunit alpha, IL23A ELISA Kit

interleukin 23, alpha subunit p19

Pig Interleukin-23 subunit alpha (IL23A) ELISA kit; IL-23; IL-23A; IL23P19; MGC79388; P19; SGRF; JKA3 induced upon T-cell activation; interleukin 23 p19 subunit; interleukin 23; alpha subunit p19

interleukin-23 subunit alpha; Interleukin-23 subunit alpha; interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin 23 p19 subunit; interleukin-23 p19 subunit; interleukin-23 subunit p19; Interleukin-23 subunit p19; IL-23p19

IL23A

IL23A; IL23A; SGRF; il23p19; SGRF; IL-23 subunit alpha; IL-23-A; IL-23p19

IL23A_PIG

Pig

193

This assay has high sensitivity and excellent specificity for detection of pig IL23A. No significant cross-reactivity or interference between pig IL23A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of pig IL23A is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL23A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL23A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL23A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL23A bound in the initial step. The color development is stopped and the intensity of the color is measured.

194332506

NP_001123708.1

NM_001130236.1

Q9N2H9

21,132 Da

Cytokine-cytokine Receptor Interaction Pathway (84463); Cytokine-cytokine Receptor Interaction Pathway (460); Inflammatory Bowel Disease (IBD) Pathway (842783); Inflammatory Bowel Disease (IBD) Pathway (842797); Jak-STAT Signaling Pathway (84489); Jak-STAT Signaling Pathway (488); Pertussis Pathway (218109); Pertussis Pathway (218099); Rheumatoid Arthritis Pathway (200307); Rheumatoid Arthritis Pathway (200269)

Function: Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150