PTTG1 Antibody (N-term) | MyBioSource MBS9201288 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PTTG1 Antibody (N-term)

catalog :

MBS9201288

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Immunofluorescent analysis of U251 cells, using PTTG1 Antibody (N-term). MBS9201288 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).

image 2 :

Formalin-fixed and paraffin-embedded human testis tissue with PTTG1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

image 3 :

Anti-PTTG1 Antibody (N-term) at 1:500 dilution + Daudi whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9201288

products type :

Antibody

products full name :

PTTG1 Antibody (N-term)

products short name :

[PTTG1]

products name syn :

[Securin; Esp1-associated protein; Pituitary tumor-transforming gene 1 protein; Tumor-transforming protein 1; hPTTG; PTTG1; EAP1; PTTG; TUTR1]

other names :

[securin; Securin; securin; pituitary tumor-transforming 1; Esp1-associated protein; Pituitary tumor-transforming gene 1 protein; Tumor-transforming protein 1; hPTTG]

products gene name :

[PTTG1]

other gene names :

[PTTG1; PTTG1; EAP1; PTTG; HPTTG; TUTR1; EAP1; PTTG; TUTR1; Tumor-transforming protein 1; hPTTG]

uniprot entry name :

PTTG1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[16-45]

sequence length :

202

specificity :

This PTTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human PTTG1.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

app notes :

IF~~1:100. WB~~1:500. IHC-P~~1:50~100

image1 heading :

Immunofluorescence (IF)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Western Blot

other info1 :

Function: Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.

other info2 :

Cellular Location: Cytoplasm. Nucleus. Tissue Location: Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.

products categories :

Crown Antibodies; Cancer; Cell Biology

products description :

PTTG1 is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The protein has transforming activity in vitro and tumorigenic activity in vivo, and is highly expressed in various tumors. This protein contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the protein can act as a transactivation domain. It is mainly a cytosolic protein, although it partially localizes in the nucleus.

products references :

Li,T., J Genet Genomics 36 (6), 335-342 (2009). Yan,S., Cancer Res. 69 (8), 3283-3290 (2009). Chesnokova,V., Horm. Res. 71 SUPPL 2, 82-87 (2009)

ncbi gi num :

538260592

ncbi acc num :

NP_001269311.1

ncbi gb acc num :

NM_001282382.1

uniprot acc num :

O95997

ncbi summary :

The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]

uniprot summary :

Securin: Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation. Belongs to the securin family. Protein type: Cell cycle regulation; Oncoprotein. Chromosomal Location of Human Ortholog: 5q35.1. Cellular Component: cytoplasm; cytosol; nucleus. Molecular Function: protein binding; cysteine protease inhibitor activity; SH3 domain binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; mitosis; regulation of transcription, DNA-dependent; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; spermatogenesis; mitotic cell cycle; DNA repair; chromosome organization and biogenesis; chromosome segregation

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage