Phospho-CDC25A (S178) Antibody | MyBioSource MBS9200987 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Phospho-CDC25A (S178) Antibody

catalog :

MBS9200987

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

The anti-Phospho-CDC25A-S178 Pab is used in Western blot to detect Phospho-CDC25A-S178 in mouse kidney tissue lysate

image 2 :

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

product information

catalog number :

MBS9200987

products type :

Antibody

products full name :

Phospho-CDC25A (S178) Antibody

products short name :

[Phospho-CDC25A (S178)]

products name syn :

[M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A; CDC25A]

other names :

[M-phase inducer phosphatase 1 isoform a; M-phase inducer phosphatase 1; M-phase inducer phosphatase 1; cell division cycle 25A; Dual specificity phosphatase Cdc25A]

products gene name :

[CDC25A]

other gene names :

[CDC25A; CDC25A; CDC25A2]

uniprot entry name :

MPIP1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence length :

524

specificity :

This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S178 of human CDC25A.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

concentration :

0.5 mg/ml

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC-P)

app notes :

WB~~1:1000. IHC-P~~1:50~100

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Function: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

products categories :

Cancer; Cell Biology; Phospho Antibodies

products description :

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

products references :

Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004). Nemoto, K., et al., Prostate 58(1):95-102 (2004). Goloudina, A., et al., Cell Cycle 2(5):473-478 (2003). Chen, M.S., et al., Mol. Cell. Biol. 23(21):7488-7497 (2003). Chow, J.P., et al., Mol. Biol. Cell 14(10):3989-4002 (2003).

ncbi gi num :

42490758

ncbi acc num :

NP_001780.2

ncbi gb acc num :

NM_001789.2

uniprot acc num :

P30304

ncbi pathways :

Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993); Folate Biosynthesis Pathway (993)

ncbi summary :

uniprot summary :

CDC25A: a member of the MPI phosphatase family. Functions as a dosage-dependent inducer in mitotic control. Required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two splice variant isoforms have been described. Protein type: EC 3.1.3.48; Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 3p21. Cellular Component: nucleoplasm; cytosol. Molecular Function: protein binding; protein tyrosine phosphatase activity; protein kinase binding. Biological Process: cell proliferation; mitosis; response to radiation; regulation of cell cycle; cell division; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; DNA replication; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage