ACTA1/alpha-actin Antibody (C-term) | MyBioSource MBS9200947 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ACTA1/alpha-actin Antibody (C-term)

catalog :

MBS9200947

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken, cow

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

ACTA1/alpha-actin Antibody (C-term) western blot analysis in A549,RD cell line and human placenta lysates (35ug/lane).This demonstrates the ACTA1/alpha-actin antibody detected the ACTA1/alpha-actin protein (arrow).

image 2 :

ACTA1/alpha-actin Antibody (C-term) (MBS9200947)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1/alpha-actin Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

image 3 :

Confocal immunofluorescent analysis of ACTA1/alpha-actin Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

product information

catalog number :

MBS9200947

products type :

Antibody

products full name :

ACTA1/alpha-actin Antibody (C-term)

products short name :

[ACTA1/alpha-actin]

products name syn :

[ACTA1/a-actin Antibody (C-term); Actin; alpha skeletal muscle; Alpha-actin-1; ACTA1; ACTA]

other names :

[actin, alpha skeletal muscle; Actin, alpha skeletal muscle; actin, alpha skeletal muscle; actin, alpha 1, skeletal muscle; Alpha-actin-1]

products gene name :

[ACTA1]

other gene names :

[ACTA1; ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM; ACTA]

uniprot entry name :

ACTS_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Humam; (Predicted Reactivity: Xenopus, Bovine, Chicken, Mouse, Rabbit, Rat, Pig)

sequence positions :

[346-375]

sequence length :

377

specificity :

This ACTA1/Alpha-actin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the C-terminal region of human ACTA1/Alpha-actin.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC-P), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA

app notes :

WB~~1:1000. IHC-P~~1:10~50. IF~~1:10~50. FC: 1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

image4 heading :

Flow Cytometry (FC/FACS)

image4 description :

ACTA1/alpha-actin Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

other info1 :

Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Cellular Location: Cytoplasm, cytoskeleton

products categories :

Crown Antibodies; Cancer; Cell Biology

products description :

The product encoded by this gene belongs to the actin. family of proteins, which are highly conserved proteins that play a. role in cell motility, structure and integrity. Alpha, beta and. gamma actin isoforms have been identified, with alpha actins being. a major constituent of the contractile apparatus, while beta and. gamma actins are involved in the regulation of cell motility. This. actin is an alpha actin that is found in skeletal muscle. Mutations. in this gene cause nemaline myopathy type 3, congenital myopathy. with excess of thin myofilaments, congenital myopathy with cores,. and congenital myopathy with fiber-type disproportion, diseases. that lead to muscle fiber defects.

products references :

Kim, E.Y., et al. Am. J. Physiol. Renal Physiol. 299 (3), F594-F604 (2010) :. Haigh, S.E., et al. Neuromuscul. Disord. 20(6):363-374(2010). Yu, G., et al. J Clin Neurosci 17(6):766-769(2010). Yu, C.H., et al. PLoS ONE 5 (7), E11878 (2010) :. Licastro, F., et al. Curr. Pharm. Des. 16(7):783-788(2010)

ncbi gi num :

4501881

ncbi acc num :

NP_001091.1

ncbi gb acc num :

NM_001100.3

uniprot acc num :

P68133

ncbi mol weight :

42051

ncbi pathways :

Peptidoglycan Biosynthesis I (meso-diaminopimelate Containing) Pathway (58); Peptidoglycan Biosynthesis I (meso-diaminopimelate Containing) Pathway (58); Peptidoglycan Biosynthesis I (meso-diaminopimelate Containing) Pathway (58); Peptidoglycan Biosynthesis I (meso-diaminopimelate Containing) Pathway (58); Peptidoglycan Biosynthesis I (meso-diaminopimelate Containing) Pathway (58); Peptidoglycan Biosynthesis I (meso-diaminopimelate Containing) Pathway (58)

ncbi summary :

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]

uniprot summary :

ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family. Protein type: Cell development/differentiation; Cytoskeletal; Contractile; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1q42.13. Cellular Component: extracellular space; sarcomere; stress fiber; actin filament; cytosol; striated muscle thin filament; actin cytoskeleton. Molecular Function: protein binding; myosin binding; structural constituent of cytoskeleton; ADP binding; ATP binding. Biological Process: skeletal muscle fiber adaptation; muscle contraction; muscle thin filament assembly; response to mechanical stimulus; response to steroid hormone stimulus; response to lithium ion; response to extracellular stimulus; skeletal muscle fiber development; cell growth; muscle filament sliding. Disease: Nemaline Myopathy 3; Myopathy, Congenital, With Fiber-type Disproportion

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage