antibody

MAPK8 Antibody (T183/Y185)

MBS9200829

0.08 mL

155 USD

polyclonal

rabbit

nonconjugated

human, rat

western blot, ELISA, enzyme immunoassay, immunohistochemistry - paraffin section

Antibody

MAPK8 Antibody (T183/Y185)

MAPK8

Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; MAPK8; JNK1; PRKM8; SAPK1; SAPK1C

mitogen-activated protein kinase 8 JNK1 beta2; Mitogen-activated protein kinase 8; mitogen-activated protein kinase 8; mitogen-activated protein kinase 8; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1

MAPK8

MAPK8; MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2; JNK1; PRKM8; SAPK1; SAPK1C; MAP kinase 8; MAPK 8; SAPK1c

MK08_HUMAN

Polyclonal

Rabbit Ig

Rabbit

Human (Predicted Reactivity: Mouse, Rat, Xenopus)

159-195

195

This MAPK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-195 amino acids from human MAPK8.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.45

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

ELISA (EIA), Immunohistochemistry-Paraffin(IHC-P), Western Blot (WB)

WB~~1:1000. IHC-P~~1:50~100

Antigen Type: Synthetic Peptide. Other Accession: P49187, Q61831, P53779, Q8QHK8, P49185, Q91Y86, NP_620637

Antigen Source: HUMAN. Cellular Location: Cytoplasm. Nucleus

Cancer; Immunology; Neuroscience; Signal Transduction

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Dasgupta, J., et al. J. Cell. Physiol. 225(1):52-62(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Gao, D., et al. J. Biol. Chem. 285(39):29965-29973(2010). Han, J.S., et al. Anticancer Res. 30(9):3407-3412(2010). Yang, X.H., et al. World J. Gastroenterol. 16(27):3385-3393(2010)

513788279

NP_001265476.1

NM_001278547.1

P45983

48296

ABC Transporters Pathway (5599); ABC Transporters Pathway (5599); ABC Transporters Pathway (5599); ABC Transporters Pathway (5599); ABC Transporters Pathway (5599); ABC Transporters Pathway (5599); ABC Transporters Pathway (5599); ABC Transporters Pathway (5599); ABC Transporters Pathway (5599); ABC Transporters Pathway (5599)

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jun 2013]

JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described. Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.24; Kinase, protein; Protein kinase, CMGC; CMGC group; MAPK family; MAPK/JNK subfamily; JNK subfamily. Chromosomal Location of Human Ortholog: 10q11.22. Cellular Component: nucleoplasm; mitochondrion; nucleus; cytosol. Molecular Function: protein serine/threonine kinase activity; protein binding; enzyme binding; histone deacetylase regulator activity; histone deacetylase binding; JUN kinase activity; ATP binding. Biological Process: nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; stress-activated MAPK cascade; rhythmic process; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of protein localization; regulation of transcription factor activity; JNK cascade; response to stress; negative regulation of protein binding; toll-like receptor 4 signaling pathway; response to UV; regulation of histone deacetylation; ossification; MyD88-independent toll-like receptor signaling pathway; peptidyl-threonine phosphorylation; regulation of circadian rhythm; toll-like receptor 2 signaling pathway; JUN phosphorylation; MyD88-dependent toll-like receptor signaling pathway; peptidyl-serine phosphorylation; response to cadmium ion; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; negative regulation of apoptosis

0.08 mL

155 USD

0.4 mL

340