ANGPTL4 Antibody (Center) | MyBioSource MBS9200783 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ANGPTL4 Antibody (Center)

catalog :

MBS9200783

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ANGPTL4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

image 2 :

Overlay histogram showing A549 cells stained with MBS9200783 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP9207c, 1:25 dilution) for 60 min at37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

image 3 :

All lanes : Anti-ANGPTL4 Antibody (Center) at 1:1000 dilution Lane 1: human skeletal muscle lysate Lane 2: human liver lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9200783

products type :

Antibody

products full name :

ANGPTL4 Antibody (Center)

products short name :

[ANGPTL4]

products name syn :

[Angiopoietin-related protein 4; Angiopoietin-like protein 4; Hepatic fibrinogen/angiopoietin-related protein; HFARP; ANGPTL4; ARP4; HFARP; PGAR]

other names :

[angiopoietin-related protein 4 isoform b; Angiopoietin-related protein 4; angiopoietin-related protein 4; angiopoietin-like 4; Angiopoietin-like protein 4; Hepatic fibrinogen/angiopoietin-related protein; HFARP]

products gene name :

[ANGPTL4]

other gene names :

[ANGPTL4; ANGPTL4; NL2; ARP4; FIAF; HARP; PGAR; HFARP; TGQTL; UNQ171; pp1158; ANGPTL2; ARP4; HFARP; PGAR; HFARP]

uniprot entry name :

ANGL4_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse

sequence positions :

[138-167]

sequence length :

368

specificity :

This ANGPTL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the Central region of human ANGPTL4.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column,followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

FC, WB, IHC-P,E

app notes :

FC~~1:25. WB~~1:1000. IHC-P~~1:50~100

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Testing Data (TD)

image3 heading :

Testing Data (TD)

other info1 :

Tissue Location: Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. Function: rotein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation (By similarity). Cellular Location: Secreted. Secreted, extracellular space,extracellular matrix Note=The unprocessed form interacts with the extracellular matrix This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4 (By similarity).

products categories :

Cancer; Cardiovascular; Metabolism; Signal Transduction

products description :

ANGPTL4 is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This protein is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. The encoded protein is a serum hormone directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity and also acts as an apoptosis survival factor for vascular endothelial cells. The encoded protein may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes.

products references :

Maxwell,T.J., et.al., Int J Mol Sci 11 (1), 370-385 (2010). Legry,V., et.al, J. Clin. Endocrinol. Metab. 94 (12), 5070-5077 (2009). Sonnenburg,W.K., et.al, J. Lipid Res. 50 (12), 2421-2429 (2009)

ncbi gi num :

89264696

ncbi acc num :

NP_001034756.1

ncbi gb acc num :

NM_001039667.2

uniprot acc num :

Q9BY76

ncbi mol weight :

45214

ncbi pathways :

Ascorbate And Aldarate Metabolism Pathway (51129); Ascorbate And Aldarate Metabolism Pathway (51129); Ascorbate And Aldarate Metabolism Pathway (51129); Ascorbate And Aldarate Metabolism Pathway (51129); Ascorbate And Aldarate Metabolism Pathway (51129); Ascorbate And Aldarate Metabolism Pathway (51129); Ascorbate And Aldarate Metabolism Pathway (51129); Ascorbate And Aldarate Metabolism Pathway (51129); Ascorbate And Aldarate Metabolism Pathway (51129)

ncbi summary :

This gene encodes a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. This protein can also act as an apoptosis survival factor for vascular endothelial cells and can prevent metastasis by inhibiting vascular growth and tumor cell invasion. The C-terminal domain may be proteolytically-cleaved from the full-length secreted protein. Decreased expression of this gene has been associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4. [provided by RefSeq, Sep 2013]

uniprot summary :

ANGPTL4: Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation. Protein type: Secreted; Inhibitor; Extracellular matrix; Secreted, signal peptide; Apoptosis. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region. Molecular Function: enzyme inhibitor activity; protein binding. Biological Process: positive regulation of angiogenesis; response to hypoxia; negative regulation of lipoprotein lipase activity; cellular lipid metabolic process; angiogenesis; cell differentiation; protein homooligomerization; negative regulation of apoptosis. Disease: Plasma Triglyceride Level Quantitative Trait Locus

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage