product summary
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company name :
MyBioSource
product type :
antibody
product name :
PJA1 Antibody (N-term)
catalog :
MBS9200719
quantity :
0.08 mL
price :
165 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, enzyme immunoassay, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
PJA1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PJA1 antibody detected the PJA1 protein (arrow).
image 2 :
PJA1 Antibody (N-term) (MBS9200719)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PJA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
product information
catalog number :
MBS9200719
products type :
Antibody
products full name :
PJA1 Antibody (N-term)
products short name :
[PJA1]
products name syn :
[E3 ubiquitin-protein ligase Praja-1; Praja1; 632-; RING finger protein 70; PJA1; RNF70]
other names :
[E3 ubiquitin-protein ligase Praja-1 isoform b; E3 ubiquitin-protein ligase Praja-1; E3 ubiquitin-protein ligase Praja-1; praja ring finger 1, E3 ubiquitin protein ligase; RING finger protein 70]
products gene name :
[PJA1]
other gene names :
[PJA1; PJA1; RNF70; PRAJA1; RNF70; Praja1]
uniprot entry name :
PJA1_HUMAN
clonality :
Polyclonal
isotype :
Rabbit Ig
host :
Rabbit
reactivity :
Human
sequence positions :
[155-183]
sequence length :
588
specificity :
This PJA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-183 amino acids from the N-terminal region of human PJA1.
purity :
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
form :
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
storage stability :
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
tested application :
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
app notes :
WB~~1:1000. IHC-P~~1:10~50
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
other info1 :
Antigen Type: Synthetic Peptide. Clone Names: RB34370
other info2 :
Antigen Source: HUMAN. Function: Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome (By similarity). May be involved in protein sorting. Tissue Location: Expressed in various regions of the brain including the cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Highest levels in the cerebral cortex.
products categories :
Cell Biology
products description :
This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked mental retardation disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants.
products references :
Wieland, I., et al. Clin. Genet. 72(6):506-516(2007). Saha, T., et al. Oncogene 25(5):693-705(2006). Mishra, L., et al. Cancer Biol. Ther. 4(7):694-699(2005). Sasaki, A., et al. J. Biol. Chem. 277(25):22541-22546(2002). Yu, P., et al. Genomics 79(6):869-874(2002)
ncbi gi num :
74048537
ncbi acc num :
NP_001027568.1
ncbi gb acc num :
NM_001032396.2
uniprot acc num :
Q8NG27
ncbi mol weight :
71002
ncbi summary :
This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked mental retardation disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
uniprot summary :
PJA1: Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome. May be involved in protein sorting. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 6.3.2.19; Ubiquitin ligase; Ligase; EC 6.3.2.-; Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: Xq13.1. Cellular Component: cytoplasm. Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity. Biological Process: protein ubiquitination; protein catabolic process
size1 :
0.08 mL
price1 :
165 USD
size2 :
0.4 mL
price2 :
370
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
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