Phospho-TBK (S172) Antibody | MyBioSource MBS9200702 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Phospho-TBK (S172) Antibody

catalog :

MBS9200702

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[RB16573]

reactivity :

human, mouse

application :

ELISA, enzyme immunoassay, dot blot

more info or order :

MyBioSource product webpage

image

image 1 :

Dot blot analysis of anti-Phospho-TBK-pS172 Antibody (MBS9200702) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

product information

catalog number :

MBS9200702

products type :

Antibody

products full name :

Phospho-TBK (S172) Antibody

products short name :

[Phospho-TBK (S172)]

products name syn :

[Serine/threonine-protein kinase TBK1; NF-kappa-B-activating kinase; T2K; TANK-binding kinase 1; TBK1; NAK]

other names :

[serine/threonine-protein kinase TBK1; Serine/threonine-protein kinase TBK1; serine/threonine-protein kinase TBK1; TANK-binding kinase 1; NF-kappa-B-activating kinase; T2K; TANK-binding kinase 1]

products gene name :

[TBK1]

other gene names :

[TBK1; TBK1; NAK; T2K; NAK]

uniprot entry name :

TBK1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

clone :

[RB16573]

host :

Rabbit

reactivity :

Human; Predicted: Zebrafish, Mouse, Xenopus

specificity :

This TBK Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S172 of human TBK.

purity :

Pepide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Dot Blot (DB), ELISA (EIA)

app notes :

DB~~1:500

image1 heading :

Dot Blot (DB)

other info1 :

Cellular Location: Cytoplasm. Note=Upon mitogen stimulation or triggering of the immune system, TBK1 is recruited to the exocyst by EXOC2. Tissue Location: Ubiquitous with higher expression in testis. Expressed in the ganglion cells, nerve fiber layer and microvasculature of the retina.

products categories :

Signal Transduction; Phospho Antibodies

products description :

Background: The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. TKB is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. The protein can form a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK. Function: Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes. Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser- 177', thus enhancing LC3 binding affinity and antibacterial autophagy (PubMed: 21617041 ). Phosphorylates SMCR8 component of the C9orf72-SMCR8 complex, promoting autophagosome maturation (PubMed: 27103069 ). Phosphorylates and activates AKT1 (PubMed: 21464307 ). Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Attenuates retroviral budding by phosphorylating the endosomal sorting complex required for transport-I (ESCRT-I) subunit VPS37C (PubMed: 21270402 ). Phosphorylates Borna disease virus (BDV) P protein (PubMed: 16155125 ). Plays an essential role in the TLR3- and IFN dependent control of herpes virus HSV-1 and HSV-2 infections in the central nervous system (PubMed: 22851595 ).

products references :

Deng,W., J. Biol. Chem. 283 (51), 35590-35597 (2008). Chessler,A.D., J. Immunol. 181 (11), 7917-7924 (2008). Soulat,D., EMBO J. 27 (15), 2135-2146 (2008)

ncbi gi num :

7019547

ncbi acc num :

NP_037386.1

ncbi gb acc num :

NM_013254.3

uniprot acc num :

Q9UHD2

ncbi summary :

The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010]

uniprot summary :

TBK1: a protein kinase of the IKK family. Can mediate NFkB activation in response to certain growth factors. Forms a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK. Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family. Chromosomal Location of Human Ortholog: 12q14.1. Cellular Component: cytoplasm; endosome membrane; cytosol. Molecular Function: protein serine/threonine kinase activity; protein binding; nucleic acid binding; phosphoprotein binding; ATP binding; protein kinase activity. Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; MyD88-independent toll-like receptor signaling pathway; response to virus; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; defense response to Gram-positive bacterium; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; positive regulation of interferon type I production; positive regulation of interferon-beta production; innate immune response; interferon type I production; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; defense response to virus; toll-like receptor 4 signaling pathway; negative regulation of interferon type I production; positive regulation of interferon-alpha production. Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 4

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage