ELISA/assay

Human HLA class II histocompatibility antigen gamma chain, CD74 ELISA Kit

MBS920059

48-Strip-Wells

510 USD

ELISA Kit

Human HLA class II histocompatibility antigen gamma chain, CD74 ELISA Kit

CD74 molecule, major histocompatibility complex, class II invariant chain

Human HLA class II histocompatibility antigen gamma chain (CD74) ELISA kit; DHLAG; HLADG; Ia-GAMMA; CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex; class II antigen-associated) ; HLA-DR-gamma; Ia-associated invariant chain; MHC HLA-DR gamma; CD74 molecule; major histocompatibility complex; class II invariant chain

HLA class II histocompatibility antigen gamma chain isoform c; HLA class II histocompatibility antigen gamma chain; HLA class II histocompatibility antigen gamma chain; p33; HLA-DR-gamma; MHC HLA-DR gamma chain; Ia-associated invariant chain; gamma chain of class II antigens; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33; CD_antigen: CD74

CD74

CD74; CD74; II; DHLAG; HLADG; Ia-GAMMA; DHLAG; Ii

HG2A_HUMAN

Human

160

This assay has high sensitivity and excellent specificity for detection of human CD74. No significant cross-reactivity or interference between human CD74 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.9 ng/ml -60 ng/ml. Sensitivity: The minimum detectable dose of human CD74 is typically less than 0.225 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD74 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD74 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD74 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD74 bound in the initial step. The color development is stopped and the intensity of the color is measured.

68448537

NP_001020329.1

NM_001025158.2

P04233

33,516 Da

Adaptive Immune System Pathway (366160); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); Immune System Pathway (106386); MHC Class II Antigen Presentation Pathway (645290); Tuberculosis Pathway (213780); Tuberculosis Pathway (213743)

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

CD74: Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. A chromosomal aberration involving CD74 is found in a non-small cell lung tumor. Results in the formation of a CD74- ROS1 chimeric protein. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, cytokine; Membrane protein, integral; Endoplasmic reticulum. Chromosomal Location of Human Ortholog: 5q32. Cellular Component: lysosomal lumen; cell surface; lysosomal membrane; integral to membrane; MHC class II protein complex; Golgi membrane; multivesicular body; membrane; plasma membrane; trans-Golgi network membrane; vacuole; intracellular; external side of plasma membrane. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; cytokine binding; beta-amyloid binding; MHC class II protein binding, via antigen binding groove; nitric-oxide synthase binding; MHC class II protein binding. Biological Process: activation of MAPK activity; positive regulation of dendritic cell antigen processing and presentation; antigen processing and presentation of exogenous peptide antigen via MHC class II; defense response; signal transduction; prostaglandin biosynthetic process; negative regulation of T cell differentiation; negative regulation of DNA damage response, signal transduction by p53 class mediator; intracellular protein transport; positive regulation of fibroblast proliferation; negative regulation of mature B cell apoptosis; positive regulation of B cell proliferation; protein complex assembly; positive thymic T cell selection; negative regulation of peptide secretion; positive regulation of T-helper 2 type immune response; chaperone cofactor-dependent protein folding; regulation of macrophage activation; T cell selection; negative thymic T cell selection; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of T cell differentiation; immunoglobulin mediated immune response; positive regulation of cytokine and chemokine mediated signaling pathway; antigen processing and presentation of endogenous antigen; negative regulation of apoptosis

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800