ULK3 Antibody (C-term) | MyBioSource MBS9200567 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ULK3 Antibody (C-term)

catalog :

MBS9200567

quantity :

0.08 mL

price :

165 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[1404CT148.89.25]

reactivity :

human, mouse, rat

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of lysates from Hela, LNCaP cell line, mouse testis and rat testis tissue lysates(from left to right), using ULK3 Antibody (C-term). MBS9200567 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

product information

catalog number :

MBS9200567

products type :

Antibody

products full name :

ULK3 Antibody (C-term)

products short name :

[ULK3]

products name syn :

[Serine/threonine-protein kinase ULK3; Unc-51-like kinase 3; ULK3]

other names :

[serine/threonine-protein kinase ULK3 isoform a; Serine/threonine-protein kinase ULK3; serine/threonine-protein kinase ULK3; unc-51 like kinase 3; Unc-51-like kinase 3]

products gene name :

[ULK3]

other gene names :

[ULK3; ULK3]

uniprot entry name :

ULK3_HUMAN

clonality :

Monoclonal

isotype :

IgG1,k

clone :

[1404CT148.89.25]

host :

Mouse

reactivity :

Human, mouse, rat

sequence length :

472

specificity :

Generated from a mouse immunized with a KLH conjugated synthetic peptide between 435-468 amino acids from the C-terminal region of human ULK3.

purity :

Purified Mouse Monoclonal Antibody (Mab)

form :

Supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB~~1:1000

image1 heading :

Western Blot (WB)

other info2 :

Cellular Location: Cytoplasm. Note=Localizes to pre-autophagosomal structure during cellular senescence. Tissue Location: Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord

products categories :

Crown Antibodies; Signal Transduction

products description :

Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.

products references :

Ota T.,et al.Nat. Genet. 36:40-45(2004). Bechtel S.,et al.BMC Genomics 8:399-399(2007). Zody M.C.,et al.Nature 440:671-675(2006). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Daub H.,et al.Mol. Cell 31:438-448(2008).

ncbi gi num :

656214624

ncbi acc num :

NP_001092906.3

ncbi gb acc num :

NM_001099436.3

uniprot acc num :

Q6PHR2

ncbi mol weight :

53444

uniprot summary :

ULK3: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, Other; Other group; ULK family. Chromosomal Location of Human Ortholog: 15q24.1. Cellular Component: membrane; cytoplasm; cytosol. Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding. Biological Process: negative regulation of smoothened signaling pathway; protein amino acid autophosphorylation; autophagy; positive regulation of smoothened signaling pathway; regulation of autophagy; autophagic vacuole formation

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage