Mouse Monoclonal Antibody (Mab) | MyBioSource MBS9200524 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Monoclonal Antibody (Mab)

catalog :

MBS9200524

quantity :

0.08 mL

price :

165 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[OKT4]

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

CD4 Antibody western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the CD4 antibody detected the CD4 protein (arrow).

product information

catalog number :

MBS9200524

products type :

Antibody

products full name :

Mouse Monoclonal Antibody (Mab)

products short name :

[CD4]

products name syn :

[T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4; CD4]

other names :

[T-cell surface glycoprotein CD4 isoform 1; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; CD4 molecule; T-cell surface antigen T4/Leu-3; CD_antigen: CD4]

products gene name :

[CD4]

other gene names :

[CD4; CD4; CD4mut]

uniprot entry name :

CD4_HUMAN

clonality :

Monoclonal

isotype :

IgG2b,k

clone :

[OKT4]

host :

Mouse

reactivity :

Human

sequence length :

458

specificity :

This CD4 monoclonal antibody is generated from mouse immunized with CD4 recombinant protein.

form :

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

concentration :

Vial Concentration: 0.47mg/ml

storage stability :

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB~~1:100~250

image1 heading :

Western Blot (WB)

other info2 :

Antigen Source: HUMAN. Function: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Cellular Location: Cell membrane; Single-pass type I membrane protein Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum

products categories :

Immunology; Signal Transduction

products description :

This gene encodes a membrane glycoprotein of T lymphocytes. that interacts with major histocompatibility complex class II. antigenes and is also a receptor for the human immunodeficiency. virus. This gene is expressed not only in T lymphocytes, but also. in B cells, macrophages, and granulocytes. It is also expressed in. specific regions of the brain. The protein functions to initiate or. augment the early phase of T-cell activation, and may function as. an important mediator of indirect neuronal damage in infectious and. immune-mediated diseases of the central nervous system. Multiple. alternatively spliced transcript variants encoding different. isoforms have been identified in this gene.

products references :

Pourgheysari, B., et al. Blood 116(16):2968-2974(2010). Rudd, C.E., et al. J. Immunol. 185(5):2645-2649(2010). Ammirati, E., et al. Arterioscler. Thromb. Vasc. Biol. 30(9):1832-1841(2010). Schenkel, J.M., et al. J. Immunol. 185(4):2013-2019(2010). Lee, K.M., et al. Am. J. Hematol. 85(8):560-563(2010)

ncbi gi num :

10835167

ncbi acc num :

NP_000607.1

ncbi gb acc num :

NM_000616.4

uniprot acc num :

P01730

ncbi pathways :

, Pathway (920); , Pathway (920); , Pathway (920); , Pathway (920); , Pathway (920); , Pathway (920); , Pathway (920); , Pathway (920); , Pathway (920); , Pathway (920)

ncbi summary :

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

uniprot summary :

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120. Protein type: Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; plasma membrane; integral to membrane; lipid raft; external side of plasma membrane. Molecular Function: protein binding; enzyme binding; transmembrane receptor activity; protein homodimerization activity; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding. Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; T cell selection; cytokine production; positive regulation of calcium-mediated signaling; defense response to Gram-negative bacterium; induction by virus of cell-cell fusion in host; signal transduction; T cell receptor signaling pathway; enzyme linked receptor protein signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; T cell costimulation; protein palmitoleylation; innate immune response; immune response; entry into host cell; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; regulation of T cell activation. Disease: Okt4 Epitope Deficiency

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage