catalog number :
MBS920045
products type :
ELISA Kit
products full name :
Human Follistatin-related protein 4, FSTL4 ELISA Kit
products short name :
follistatin-like 4
products name syn :
Human Follistatin-related protein 4 (FSTL4) ELISA kit; follistatin-like 4
other names :
follistatin-related protein 4; Follistatin-related protein 4; follistatin-related protein 4; follistatin-like protein 4; follistatin-like 4; Follistatin-like protein 4
products gene name :
FSTL4
other gene names :
FSTL4; FSTL4; KIAA1061
uniprot entry name :
FSTL4_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human FSTL4. No significant cross-reactivity or interference between human FSTL4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of human hollistatin-related protein 4 (FSTL4) concentrations in serum, plasma, tissue homogenates. Detection Range: 1.56 ng/ml-100 ng/ml.
other info2 :
Sensitivity: The minimum detectable dose of human FSTL4 is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FSTL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSTL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FSTL4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSTL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_055897.1
ncbi gb acc num :
NM_015082.1
ncbi mol weight :
93,096 Da