CD40 Antibody (C-term) (Ascites) | MyBioSource MBS9200224 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD40 Antibody (C-term) (Ascites)

catalog :

MBS9200224

quantity :

0.1 mL

price :

370 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[647CT13.2.4]

reactivity :

human, mouse

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

CD40 Antibody (C-term)(Ascites) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CD40 antibody detected the CD40 protein (arrow).

product information

catalog number :

MBS9200224

products type :

Antibody

products full name :

CD40 Antibody (C-term) (Ascites)

products short name :

[CD40]

products name syn :

[Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; CD40; CD40; TNFRSF5]

other names :

[tumor necrosis factor receptor superfamily member 5 isoform 1; Tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily member 5; CD40 molecule, TNF receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; CD_antigen: CD40]

products gene name :

[CD40]

other gene names :

[CD40; CD40; p50; Bp50; CDW40; TNFRSF5; TNFRSF5]

uniprot entry name :

TNR5_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[647CT13.2.4]

host :

Mouse

reactivity :

Human (Predicted Reactivity: Horse, Mouse, Pig)

sequence positions :

[247-276]

sequence length :

277

specificity :

This CD40 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 247-276 amino acids from the C-terminal region of human CD40.

form :

Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB~~1:100~1600

image1 heading :

Western Blot (WB)

other info1 :

. Calculated MW: 30619. Antigen Region: 247-276

other info2 :

Antigen Source: HUMAN. Function: Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. Cellular Location: Isoform I: Cell membrane; Single-pass type I membrane protein. Tissue Location: B-cells and in primary carcinomas.

products categories :

Cardiovascular; Immunology; Signal Transduction

products description :

The protein encoded by this gene is a member of the. TNF-receptor superfamily. This receptor has been found to be. essential in mediating a broad variety of immune and inflammatory. responses including T cell-dependent immunoglobulin class. switching, memory B cell development, and germinal center. formation. AT-hook transcription factor AKNA is reported to. coordinately regulate the expression of this receptor and its. ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a. mediator of the signal transduction. The interaction of this. receptor and its ligand is found to be necessary for. amyloid-beta-induced microglial activation, and thus is thought to. be an early event in Alzheimer disease pathogenesis. Two. alternatively spliced transcript variants of this gene encoding. distinct isoforms have been reported.

products references :

Rodriguez-Rodriguez, L., et al. J. Rheumatol. 37(10):2076-2080(2010). Lewis, J.P., et al. Genomics 96(4):211-219(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Blanco-Kelly, F., et al. PLoS ONE 5 (7), E11520 (2010) :. Soliman, M.A., et al. Egypt J Immunol 16(1):61-70(2009)

ncbi gi num :

4507581

ncbi acc num :

NP_001241.1

ncbi gb acc num :

NM_001250.5

uniprot acc num :

P25942

ncbi pathways :

Cell Cycle - Yeast Pathway (958); Cell Cycle - Yeast Pathway (958); Cell Cycle - Yeast Pathway (958); Cell Cycle - Yeast Pathway (958); Cell Cycle - Yeast Pathway (958); Cell Cycle - Yeast Pathway (958); Cell Cycle - Yeast Pathway (958); Cell Cycle - Yeast Pathway (958); Cell Cycle - Yeast Pathway (958); Cell Cycle - Yeast Pathway (958)

ncbi summary :

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]

uniprot summary :

CD40: a member of the TNF-receptor superfamily. This receptor for CD40L mediates a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. Defects in CD40 are the cause of hyper-IgM immunodeficiency type 3 (HIGM3). HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. Two alternatively spliced isoforms have been reported. Isoform I is a type I membrane protein; isoform II is secreted. Protein type: Membrane protein, integral; Receptor, cytokine. Chromosomal Location of Human Ortholog: 20q12-q13.2. Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane. Molecular Function: protein binding; signal transducer activity; enzyme binding; ubiquitin protein ligase binding; receptor activity; antigen binding. Biological Process: B cell proliferation; platelet activation; positive regulation of isotype switching to IgG isotypes; regulation of immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; immune response-regulating cell surface receptor signaling pathway; activation of NF-kappaB transcription factor; cellular calcium ion homeostasis; positive regulation of MAP kinase activity; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of B cell proliferation; protein kinase B signaling cascade; protein complex assembly; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; inflammatory response; defense response to virus; regulation of immunoglobulin secretion. Disease: Immunodeficiency With Hyper-igm, Type 3

size1 :

0.1 mL

price1 :

370 USD

more info or order :

MyBioSource product webpage