LC3 Antibody (APG8) | MyBioSource MBS9200215 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

LC3 Antibody (APG8)

catalog :

MBS9200215

quantity :

0.08 mL

price :

165 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[166AT1234]

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-MAP1LC3A Antibody at 1:5000 dilution + recombinant protein Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

All lanes : Anti-MAP1LC3A Antibody at 1:2000 dilution Lane 1: human brain lysates Lane 2: mouse brain lysates Lane 3: rat brain lysates Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image 3 :

Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8). U251 cells(right) were treated with Chloroquine (50 uM,16h). MBS9200215 was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

product information

catalog number :

MBS9200215

products type :

Antibody

products full name :

LC3 Antibody (APG8)

products short name :

[LC3]

products name syn :

[Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha; MAP1LC3A]

other names :

[microtubule-associated proteins 1A/1B light chain 3A isoform a; Microtubule-associated proteins 1A/1B light chain 3A; microtubule-associated proteins 1A/1B light chain 3A; microtubule-associated protein 1 light chain 3 alpha; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha]

products gene name :

[MAP1LC3A]

other gene names :

[MAP1LC3A; MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3; MAP1A/MAP1B LC3 A]

uniprot entry name :

MLP3A_HUMAN

clonality :

Monoclonal

isotype :

Mouse IgG1 k

clone :

[166AT1234]

host :

Mouse

reactivity :

Human, mouse, rat

sequence length :

121

specificity :

This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).

purity :

Purified Mouse Monoclonal Antibody (Mab)

form :

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

concentration :

Vial Concentration: 0.5

storage stability :

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC) - Paraffin, Immunofluorescence (IF)

app notes :

WB~~1:1000. IF~~1:200. IHC-P~~1:50~100

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunofluorescence (IF)

image4 heading :

Immunofluorescence (IF)

image4 description :

image5 heading :

Immunofluorescence (IF)

image5 description :

Fluorescent image of U251 cells stained with MBS9200215 LC3 (APG8) antibody.U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9200215 LC3 (APG8) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

other info2 :

Antigen Source: HUMAN

products categories :

Signal Transduction; Neuroscience; Microbiology; Metabolism; Cell Biology; Cardiovascular; Cancer; Autophagy

products description :

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.

products references :

References for protein:. 1.Autophagy negatively regulates Wnt signalling by promoting Dishevelled degradation. Gao C, et al. Nat Cell Biol, 2010 Aug. PMID 20639871. 2.The prolyl isomerase Pin1 induces LC-3 expression and mediates tamoxifen resistance in breast cancer. Namgoong GM, et al. J Biol Chem, 2010 Jul 30. PMID 20479004. 3.Protein kinase C inhibits autophagy and phosphorylates LC3. Jiang H, et al. Biochem Biophys Res Commun, 2010 May 14. PMID 20398630. 4. Processing of autophagic protein LC3 by the 20S proteasome. Gao Z, et al. Autophagy, 2010 Jan. PMID 20061800. 5.Defective autophagy associated with LC3 puncta in epothilone-resistant cancer cells. Shen S, et al. Cell Cycle, 2010 Jan 15. PMID 20023420. References for U251 cell line:. 1. Westermark B.; Pontén J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449]. 2. Pontén, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950]. 3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].

ncbi gi num :

14210522

ncbi acc num :

NP_115903.1

ncbi gb acc num :

NM_032514.3

uniprot acc num :

Q9H492

ncbi mol weight :

14272

ncbi pathways :

, Pathway (84557)

ncbi summary :

uniprot summary :

LC3A: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Two isoforms of the human protein are produced by alternative splicing. Protein type: Microtubule-binding; Ubiquitin-like modifier; Vesicle; Autophagy. Chromosomal Location of Human Ortholog: 20q11.22. Cellular Component: microtubule; extrinsic to membrane; late endosome; autophagic vacuole; cytoplasmic vesicle; cytosol. Molecular Function: protein binding; phosphatidylethanolamine binding; microtubule binding; GABA receptor binding; phospholipid binding. Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage