ITA6 Antibody | MyBioSource MBS9200194 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ITA6 Antibody

catalog :

MBS9200194

quantity :

0.08 mL

price :

165 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[129CT49.6.5]

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-ITA6 at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 127 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

ITA6 Monoclonal Antibody (Cat. #MBS9200194) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conj?gation of the secondary antibody and DAB staining. This data demonstrates the use of the ITA6 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

image 3 :

Confocal immunofluorescent analysis of ITA6 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

product information

catalog number :

MBS9200194

products type :

Antibody

products full name :

ITA6 Antibody

products short name :

[ITA6]

products name syn :

[Integrin alpha-6; CD49 antigen-like family member F; VLA-6; CD49f; Integrin alpha-6 heavy chain; Integrin alpha-6 light chain; Processed integrin alpha-6; Alpha6p; ITGA6]

other names :

[integrin alpha-6 isoform b; Integrin alpha-6; integrin alpha-6; integrin, alpha 6; CD49 antigen-like family member F; VLA-6; CD_antigen: CD49f]

products gene name :

[ITGA6]

other gene names :

[ITGA6; ITGA6; CD49f; VLA-6; ITGA6B; Alpha6p]

uniprot entry name :

ITA6_HUMAN

clonality :

Monoclonal

isotype :

IgG1,kappa

clone :

[129CT49.6.5]

host :

Mouse

reactivity :

Human

sequence length :

1073

specificity :

ITA6 recombinant protein is used to produce this monoclonal antibody.

purity :

Purified Mouse Monoclonal Antibody (Mab)

form :

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

concentration :

Vial Concentration: 0.42

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry-Paraffin (IHC-P)

app notes :

WB~~1:1000. IHC-P~~1:10~50. IF~~1:10~50. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry

image3 heading :

Immunofluorescence (IF)

image4 heading :

Flow Cytometry (FC/FACS)

image4 description :

ITA6 Monoclonal Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

other info1 :

Antigen Type: Recombinant Protein

other info2 :

Antigen Source: HUMAN. Cellular Location: Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Tissue Location: Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).

products categories :

Developmental Biology; Signal Transduction

products description :

The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene.

products references :

Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility hot-spot. Johnatty SE, et al. PLoS Genet, 2010 Jul 8. PMID 20628624. Prostate cancer risk-associated variants reported from genome-wide association studies: Meta-analysis and their contribution to genetic Variation. Kim ST, et al. Prostate, 2010 Jun 16. PMID 20564319. alpha6-integrin subunit plays a major role in the proangiogenic properties of endothelial progenitor cells. Bouvard C, et al. Arterioscler Thromb Vasc Biol, 2010 Aug. PMID 20508204. Common polymorphisms in ITGA2, PON1 and THBS2 are associated with coronary atherosclerosis in a candidate gene association study of the Chinese Han population. Wang Y, et al. J Hum Genet, 2010 Aug. PMID 20485444. Usage of heparan sulfate, integrins, and FAK in HPV16 infection. Abban CY, et al. Virology, 2010 Jul 20. PMID 20441998.

ncbi gi num :

119395742

ncbi acc num :

NP_000201.2

ncbi gb acc num :

NM_000210.2

uniprot acc num :

P23229

ncbi mol weight :

126606

ncbi summary :

uniprot summary :

ITGA6: Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with HPS5. Interacts with RAB21. Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). Belongs to the integrin alpha chain family. 8 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis; Cell adhesion. Chromosomal Location of Human Ortholog: 2q31.1. Cellular Component: focal adhesion; cell surface; cell-cell adherens junction; hemidesmosome; basal plasma membrane; plasma membrane; basement membrane; filopodium; external side of plasma membrane. Molecular Function: integrin binding; protein binding; metal ion binding; laminin binding. Biological Process: integrin-mediated signaling pathway; skin development; extracellular matrix organization and biogenesis; filopodium formation; positive regulation of apoptosis; cell-matrix adhesion; cell-substrate adhesion; hemidesmosome assembly; cell-cell adhesion; gut development; cellular response to extracellular stimulus; brown fat cell differentiation; cell-substrate junction assembly; positive regulation of cell-cell adhesion; cell adhesion mediated by integrin; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; leukocyte migration; positive regulation of phosphorylation. Disease: Epidermolysis Bullosa Junctionalis With Pyloric Atresia

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage