TLR3 Antibody | MyBioSource MBS9200129 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

TLR3 Antibody

catalog :

MBS9200129

quantity :

0.08 mL

price :

165 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[303CT19.9.2]

reactivity :

human, mouse

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-TLR3 Antibody at 1:500 dilution + K562 whole cell lysates. Lysates/proteins at 20 ug per lane. Secondary. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 104 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

TLR3 Antibody western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the TLR3 antibody detected the TLR3 protein (arrow).

product information

catalog number :

MBS9200129

products type :

Antibody

products full name :

TLR3 Antibody

products short name :

[TLR3]

products name syn :

[Toll-like receptor 3; CD283; TLR3]

other names :

[toll-like receptor 3; Toll-like receptor 3; toll-like receptor 3; toll-like receptor 3; CD_antigen: CD283]

products gene name :

[TLR3]

other gene names :

[TLR3; TLR3; CD283; IIAE2]

uniprot entry name :

TLR3_HUMAN

clonality :

Monoclonal

isotype :

IgM,k

clone :

[303CT19.9.2]

host :

Mouse

reactivity :

Human, mouse

sequence length :

904

specificity :

This TLR3 monoclonal antibody is generated from mouse immunized with TLR3 recombinant protein.

form :

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB~~1:100

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

other info1 :

Function: Key component of innate and adaptiveimmunity. TLRs (Toll-like receptors) controlhost immune response against pathogensthrough recognition of molecular patterns specific to microorganisms. TLR3 is anucleotide-sensing TLR which is activated bydouble-stranded RNA, a sign of viral infection.Acts via the adapter TRIF/TICAM1, leading toNF-kappa-B activation, IRF3 nucleartranslocation, cytokine secretion and theinflammatory response. Cellular Location: Endoplasmic reticulum membrane;Single-pass type I membrane protein.Endosome membrane. Early endosome. Tissue Location: Expressed at high level in placenta andpancreas. Also detected in CD11c+ immaturedendritic cells. Only expressed in dendriticcells and not in other leukocytes, includingmonocyte precursors. TLR3 is the TLR that isexpressed most strongly in the brain,especially in astrocytes, glia, and neurons.

products categories :

Crown Antibodies; Immunology; Neuroscience

products description :

The protein encoded by this gene is a member of the. Toll-like receptor (TLR) family which plays a fundamental role in. pathogen recognition and activation of innate immunity. TLRs are. highly conserved from Drosophila to humans and share structural and. functional similarities. They recognize pathogen-associated. molecular patterns (PAMPs) that are expressed on infectious agents,. and mediate the production of cytokines necessary for the. development of effective immunity. The various TLRs exhibit. different patterns of expression. This receptor is most abundantly. expressed in placenta and pancreas, and is restricted to the. dendritic subpopulation of the leukocytes. It recognizes dsRNA. associated with viral infection, and induces the activation of. NF-kappaB and the production of type I interferons. It may thus. play a role in host defense against viruses. Use of alternative. polyadenylation sites to generate different length transcripts has. been noted for this gene.

products references :

Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :. Perez de Diego, R., et al. Immunity 33(3):400-411(2010). Zhou, Y., et al. Immunology 131(1):40-49(2010). Perrot, I., et al. J. Immunol. 185(4):2080-2088(2010). Paone, A., et al. Neoplasia 12(7):539-549(2010)

ncbi gi num :

4507531

ncbi acc num :

NP_003256.1

ncbi gb acc num :

NM_003265.2

uniprot acc num :

O15455

ncbi mol weight :

103829

ncbi pathways :

Fatty Acid Degradation Pathway (7098); Fatty Acid Degradation Pathway (7098); Fatty Acid Degradation Pathway (7098); Fatty Acid Degradation Pathway (7098); Fatty Acid Degradation Pathway (7098); Fatty Acid Degradation Pathway (7098); Fatty Acid Degradation Pathway (7098); Fatty Acid Degradation Pathway (7098); Fatty Acid Degradation Pathway (7098); Fatty Acid Degradation Pathway (7098)

ncbi summary :

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

TLR3: a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. Most abundantly expressed in placenta and pancreas. Restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection. Acts via MyD88 and TRAF6, leading to the activation of NF-kappaB and the production of type I interferon. Protein type: Membrane protein, integral; Receptor, cytokine. Chromosomal Location of Human Ortholog: 4q35. Cellular Component: Golgi membrane; endoplasmic reticulum membrane; cell surface; membrane; lysosomal membrane; integral to plasma membrane; cytoplasm; endosome membrane. Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; double-stranded RNA binding; receptor activity. Biological Process: negative regulation of osteoclast differentiation; I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; positive regulation of apoptosis; positive regulation of JNK cascade; positive regulation of interferon-alpha biosynthetic process; positive regulation of NF-kappaB import into nucleus; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to exogenous dsRNA; positive regulation of interferon-beta production; inflammatory response; defense response to virus; toll-like receptor 4 signaling pathway; MyD88-independent toll-like receptor signaling pathway; male gonad development; activation of NF-kappaB-inducing kinase; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine production; hyperosmotic response; positive regulation of chemokine biosynthetic process; detection of virus; defense response to bacterium; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; I-kappaB phosphorylation; microglial cell activation during immune response; positive regulation of inflammatory response; positive regulation of interferon-gamma biosynthetic process. Disease: Human Immunodeficiency Virus Type 1, Susceptibility To

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage