PAH Antibody (Center) | MyBioSource MBS9200125 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PAH Antibody (Center)

catalog :

MBS9200125

quantity :

0.08 mL

price :

165 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[717CT21.1.1]

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded H. liver section using PAH Antibody (Center). MBS9200125 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

image 2 :

MBS9200125 staining PAH in human kidney tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

image 3 :

Immunohistochemical analysis of paraffin-embedded M. kidney section using PAH Antibody (Center). MBS9200125 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

product information

catalog number :

MBS9200125

products type :

Antibody

products full name :

PAH Antibody (Center)

products short name :

[PAH]

products name syn :

[Phenylalanine-4-hydroxylase; PAH; Phe-4-monooxygenase; PAH]

other names :

[phenylalanine-4-hydroxylase; Phenylalanine-4-hydroxylase; phenylalanine-4-hydroxylase; phenylalanine hydroxylase; Phe-4-monooxygenase]

products gene name :

[PAH]

other gene names :

[PAH; PAH; PH; PKU; PKU1; PAH]

uniprot entry name :

PH4H_HUMAN

clonality :

Monoclonal

isotype :

IgG1,kappa

clone :

[717CT21.1.1]

host :

Mouse

reactivity :

Human, mouse, rat (Predicted Reactivity: Bovine)

sequence length :

452

specificity :

This PAH antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 127-161 amino acids from the Central region of human PAH.

purity :

Purified Mouse Monoclonal Antibody (Mab)

form :

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

app notes :

IHC~~1:25. WB~~1:1000

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Western Blot (WB)

image4 description :

Western blot analysis of lysates from HepG2 cell line and mouse liver rat liver tissue lysates (from left to right) using PAH Antibody (Center). MBS9200125 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

other info1 :

Calculated MW: 51862

products categories :

Crown Antibodies; Metabolism; Signal Transduction

products references :

Kwok S.C.M.,et al.Biochemistry 24:556-561(1985). Scriver C.R.,et al.Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases. Cotton R.G.,et al.Biochem. J. 255:193-196(1988). Miranda F.F.,et al.J. Biol. Chem. 277:40937-40943(2002). Siltberg-Liberles J.,et al.Gene 427:86-92(2008).

ncbi gi num :

4557819

ncbi acc num :

NP_000268.1

ncbi gb acc num :

NM_000277.1

uniprot acc num :

P00439

ncbi pathways :

Fatty Acid Biosynthesis Pathway (5053); Fatty Acid Biosynthesis Pathway (5053); Fatty Acid Biosynthesis Pathway (5053); Fatty Acid Biosynthesis Pathway (5053); Fatty Acid Biosynthesis Pathway (5053); Fatty Acid Biosynthesis Pathway (5053); Fatty Acid Biosynthesis Pathway (5053); Fatty Acid Biosynthesis Pathway (5053); Fatty Acid Biosynthesis Pathway (5053); Fatty Acid Biosynthesis Pathway (5053)

ncbi summary :

PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. [provided by RefSeq, Jul 2008]

uniprot summary :

PAH: phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. Protein type: Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Oxidoreductase; EC 1.14.16.1. Chromosomal Location of Human Ortholog: 12q22-q24.2. Cellular Component: cytosol. Molecular Function: amino acid binding; iron ion binding; phenylalanine 4-monooxygenase activity. Biological Process: L-phenylalanine catabolic process; catecholamine biosynthetic process; amino acid biosynthetic process; neurotransmitter biosynthetic process. Disease: Phenylketonuria

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage