BCL6 Antibody (C-term) (ascites) | MyBioSource MBS9200087 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

BCL6 Antibody (C-term) (ascites)

catalog :

MBS9200087

quantity :

0.1 mL

price :

370 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[305CT16.3.3]

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

BCL6 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the BCL6 antibody detected the BCL6 protein (arrow).

image 2 :

Confocal immunofluorescent analysis of BCL6 Antibody (C-term) (ascites) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

product information

catalog number :

MBS9200087

products type :

Antibody

products full name :

BCL6 Antibody (C-term) (ascites)

products short name :

[BCL6]

products name syn :

[B-cell lymphoma 6 protein; BCL-6; B-cell lymphoma 5 protein; BCL-5; Protein LAZ-3; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51; BCL6; BCL5; LAZ3; ZBTB27; ZNF51]

other names :

[B-cell lymphoma 6 protein isoform 1; B-cell lymphoma 6 protein; B-cell lymphoma 6 protein; B-cell CLL/lymphoma 6; B-cell lymphoma 5 protein; BCL-5; Protein LAZ-3; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51]

products gene name :

[BCL6]

other gene names :

[BCL6; BCL6; BCL5; LAZ3; BCL6A; ZNF51; ZBTB27; BCL5; LAZ3; ZBTB27; ZNF51; BCL-6; BCL-5]

uniprot entry name :

BCL6_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[305CT16.3.3]

host :

Mouse

reactivity :

Human

sequence positions :

[676-704]

sequence length :

706

specificity :

This BCL6 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 676-704 amino acids from the C-terminal region of human BCL6.

form :

Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunofluorescence (IF)

app notes :

WB~~1:1000~16000. IF~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Antigen Type: Synthetic Peptide

other info2 :

Antigen Source: HUMAN. Calculated MW: 78846. Function: Transcriptional repressor mainly required forgerminal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineageand biological functions. Forms complexeswith different corepressors and histonedeacetylases to repress the transcriptionalexpression of different subsets of targetgenes. Represses its target genes by bindingdirectly to the DNA sequence5'-TTCCTAGAA-3' (BCL6- binding site) orindirectly by repressing the transcriptionalactivity of transcription factors. In GC B-cells,represses genes that function indifferentiation, inflammation, apoptosis andcell cycle control, also autoregulates itstranscriptional expression and up-regulates,indirectly, the expression of some genesimportant for GC reactions, such as AICDA,through the repression of microRNAsexpression, like miR155. An importantfunction is to allow GC B-cells to proliferatevery rapidly in response to T-cell dependentantigens and tolerate the physiological DNAbreaks required for immunglobulin classswitch recombination and somatichypermutation without inducing ap53/TP53-dependent apoptotic response. Infollicular helper CD4(+) T-cells (T(FH) cells),promotes the expression of T(FH)-relatedgenes but inhibits the differentiation of T(H)1,T(H)2 and T(H)17 cells. Also required for theestablishment and maintenance ofimmunological memory for both T- andB-cells. Suppresses macrophage proliferationthrough competition with STAT5 forSTAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In responseto genotoxic stress, controls cell cycle arrestin GC B-cells in both p53/TP53- dependedentand -independent manners. Besides, alsocontrols neurogenesis through the alterationof the composition of NOTCH- dependenttranscriptional complexes at selective NOTCHtargets, such as HES5, including therecruitment of the deacetylase SIRT1 andresulting in an epigenetic silencing leading toneuronal differentiation. Cellular Location: Nucleus. Tissue Location: Expressed in germinal center T- and B-cellsand in primary immature dendritic cells

products categories :

Cancer

products description :

The protein encoded by this gene is a zinc finger. transcription factor and contains an N-terminal POZ domain. This. protein acts as a sequence-specific repressor of transcription, and. has been shown to modulate the transcription of START-dependent. IL-4 responses of B cells. This protein can interact with a variety. of POZ-containing proteins that function as transcription. corepressors. This gene is found to be frequently translocated and. hypermutated in diffuse large-cell lymphoma (DLCL), and may be. involved in the pathogenesis of DLCL. Alternatively spliced. transcript variants encoding different protein isoforms have been. found for this gene.

products references :

Chang, C.C., et al. J. Immunol. 185(10):5714-5722(2010). Yang, J., et al. J. Biol. Chem. 285(39):29760-29769(2010). Lai, A.Y., et al. J. Exp. Med. 207(9):1939-1950(2010). Ramachandrareddy, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(26):11930-11935(2010). Gomyo, H., et al. Hematology 15(3):157-161(2010)

ncbi gi num :

195927002

ncbi acc num :

NP_001124317.1

ncbi gb acc num :

NM_001130845.1

uniprot acc num :

P41182

ncbi pathways :

Pantothenate And CoA Biosynthesis Pathway (604); Pantothenate And CoA Biosynthesis Pathway (604); Pantothenate And CoA Biosynthesis Pathway (604); Pantothenate And CoA Biosynthesis Pathway (604); Pantothenate And CoA Biosynthesis Pathway (604); Pantothenate And CoA Biosynthesis Pathway (604); Pantothenate And CoA Biosynthesis Pathway (604); Pantothenate And CoA Biosynthesis Pathway (604); Pantothenate And CoA Biosynthesis Pathway (604)

ncbi summary :

The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

uniprot summary :

Bcl-6: a zinc finger transcription factor and contains an N-terminal BTB/POZ domain. A sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. Mediates transcriptional repression and interacts with components of histone deacetylase co-repressor complexes including N-CoR and SMRT. The gene is frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Protein type: Transcription factor; Oncoprotein; C2H2-type zinc finger protein. Chromosomal Location of Human Ortholog: 3q27. Cellular Component: replication fork; nucleus. Molecular Function: protein binding; chromatin DNA binding; sequence-specific DNA binding; metal ion binding; chromatin binding; transcription factor activity. Biological Process: erythrocyte development; positive regulation of apoptosis; regulation of memory T cell differentiation; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; Rho protein signal transduction; negative regulation of cell proliferation; positive regulation of histone deacetylation; negative regulation of isotype switching to IgE isotypes; germinal center formation; positive regulation of B cell proliferation; negative regulation of mast cell cytokine production; inflammatory response; negative regulation of T-helper 2 cell differentiation; regulation of immune response; T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of cell motility; negative regulation of cell-matrix adhesion; regulation of germinal center formation; negative regulation of Rho protein signal transduction; protein import into nucleus, translocation; B cell differentiation; regulation of inflammatory response; negative regulation of Notch signaling pathway; spermatogenesis; negative regulation of cell growth; positive regulation of neuron differentiation; negative regulation of transcription, DNA-dependent; actin cytoskeleton organization and biogenesis; response to DNA damage stimulus; negative regulation of B cell apoptosis

size1 :

0.1 mL

price1 :

370 USD

more info or order :

MyBioSource product webpage