catalog number :
MBS919938
products type :
ELISA Kit
products full name :
Mouse Tumor necrosis factor-inducible gene 6 protein, TNFAIP6 ELISA Kit
products short name :
tumor necrosis factor, alpha-induced protein 6
products name syn :
Mouse Tumor necrosis factor-inducible gene 6 protein (TNFAIP6) ELISA kit; TSG-6; TSG6; hyaluronate-binding protein; tumor necrosis factor alpha-inducible protein 6; tumor necrosis factor-inducible protein 6; tumor necrosis factor-stimulated gene-6 protein; tumor necrosis factor; alpha-induced protein 6
other names :
tumor necrosis factor-inducible gene 6 protein; Tumor necrosis factor-inducible gene 6 protein; tumor necrosis factor-inducible gene 6 protein; TNF alpha-induced protein 6; TNF-stimulated gene 6 protein; tumor necrosis factor induced protein 6; tumor necrosis factor alpha-induced protein 6; tumor necrosis factor, alpha-induced protein 6; tumor necrosis factor alpha induced protein 6; TNF-stimulated gene 6 protein; TSG-6; Tumor necrosis factor alpha-induced protein 6; TNF alpha-induced protein 6
products gene name :
TNFAIP6
other gene names :
Tnfaip6; Tnfaip6; Tsg6; TSG-6; Tnfip6; Tnfip6; Tsg6; TSG-6; TNF alpha-induced protein 6
uniprot entry name :
TSG6_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse TNFAIP6. No significant cross-reactivity or interference between mouse TNFAIP6 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: The minimum detectable dose of mouse TNFAIP6 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNFAIP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFAIP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFAIP6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFAIP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_033424.1
ncbi gb acc num :
NM_009398.2
ncbi mol weight :
30,924 Da
uniprot summary :
TNFAIP6: Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis. Cellular Component: extracellular space. Molecular Function: hyaluronic acid binding. Biological Process: cell adhesion; positive regulation of cell migration
size4 :
10x96-Strip-Wells