product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Sialic acid-binding Ig-like lectin 8, SIGLEC8 ELISA Kit
catalog :
MBS919455
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS919455
products type :
ELISA Kit
products full name :
Human Sialic acid-binding Ig-like lectin 8, SIGLEC8 ELISA Kit
products short name :
sialic acid binding Ig-like lectin 8
products name syn :
Human Sialic acid-binding Ig-like lectin 8 (SIGLEC8) ELISA kit; MGC59785; SAF2; SIGLEC-8; SIGLEC8L; ; sialic acid binding Ig-like lectin 8
other names :
sialic acid-binding Ig-like lectin 8; Sialic acid-binding Ig-like lectin 8; sialic acid-binding Ig-like lectin 8; SAF-2; CDw329; sialoadhesin family member 2; sialic acid binding Ig-like lectin 8; Sialoadhesin family member 2; SAF-2
products gene name :
SIGLEC8
other gene names :
SIGLEC8; SIGLEC8; SAF2; SIGLEC-8; SIGLEC8L; SAF2; Siglec-8; SAF-2
uniprot entry name :
SIGL8_HUMAN
reactivity :
Human
sequence length :
499
specificity :
This assay has high sensitivity and excellent specificity for detection of human SIGLEC8. No significant cross-reactivity or interference between human SIGLEC8 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human SIGLEC8 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SIGLEC8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SIGLEC8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIGLEC8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
94538343
ncbi acc num :
NP_055257.2
ncbi gb acc num :
NM_014442.2
uniprot acc num :
Q9NYZ4
ncbi mol weight :
54,042 Da
ncbi summary :
Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010]
uniprot summary :
SIGLEC8: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, misc.; Cell adhesion. Chromosomal Location of Human Ortholog: 19q13.33-q13.41. Cellular Component: integral to membrane. Molecular Function: protein binding; transmembrane receptor activity; carbohydrate binding. Biological Process: signal transduction; cell adhesion
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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