ELISA/assay

Human Proto-oncogene Wnt-3, WNT3 ELISA Kit

MBS919345

48-Strip-Wells

510 USD

ELISA Kit

Human Proto-oncogene Wnt-3, WNT3 ELISA Kit

wingless-type MMTV integration site family, member 3

Human Proto-oncogene Wnt-3 (WNT3) ELISA kit; INT4; MGC131950; MGC138321; MGC138323; WNT-3 proto-oncogene protein; wingless-type MMTV integration site family; member 3

proto-oncogene Wnt-3; Proto-oncogene Wnt-3; proto-oncogene Wnt-3; WNT-3 proto-oncogene protein; proto-oncogene Int-4 homolog; wingless-type MMTV integration site family, member 3; Proto-oncogene Int-4 homolog

WNT3

WNT3; WNT3; INT4; INT4

WNT3_HUMAN

Human

355

This assay has high sensitivity and excellent specificity for detection of human WNT3. No significant cross-reactivity or interference between human WNT3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human WNT3 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

13540477

NP_110380.1

NM_030753.4

P56703

39,645 Da

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Class B/2 (Secretin Family Receptors) Pathway (106378); DNA Damage Response (only ATM Dependent) Pathway (198827); GPCR Ligand Binding Pathway (161020); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83063); Hedgehog Signaling Pathway (474); Melanogenesis Pathway (83092)

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region. [provided by RefSeq, Jul 2008]

WNT3: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Defects in WNT3 are the cause of autosomal recessive tetra-amelia (ARTTRA). Tetra-amelia is a rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects. Belongs to the Wnt family. Protein type: Secreted, signal peptide; Oncoprotein; Secreted. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: proteinaceous extracellular matrix; extracellular space; endoplasmic reticulum lumen; Golgi lumen; plasma membrane; extracellular region. Molecular Function: protein domain specific binding; protein binding; frizzled binding; receptor agonist activity. Biological Process: embryonic forelimb morphogenesis; axon guidance; cell fate commitment; cell morphogenesis; dorsal/ventral axis specification; Wnt receptor signaling pathway through beta-catenin; positive regulation of collateral sprouting in the absence of injury; negative regulation of axon extension involved in axon guidance; embryonic hindlimb morphogenesis; Spemann organizer formation at the anterior end of the primitive streak; neuron differentiation; limb bud formation; mesoderm formation; gamete generation; anterior/posterior axis specification. Disease: Tetraamelia, Autosomal Recessive

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800