Human ELAV-like protein 1, ELAVL1 ELISA Kit | MyBioSource MBS919324 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human ELAV-like protein 1, ELAVL1 ELISA Kit

catalog :

MBS919324

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS919324

products type :

ELISA Kit

products full name :

Human ELAV-like protein 1, ELAVL1 ELISA Kit

products short name :

ELAV (embryonic lethal, abnormal vision, Drosophila) -like 1 (Hu antigen R)

products name syn :

Human ELAV-like protein 1 (ELAVL1) ELISA kit; ELAV1; HUR; Hua; MelG; ELAV-like 1; Hu antigen R; embryonic lethal; abnormal vision; drosophila; homolog-like 1; ELAV (embryonic lethal; abnormal vision; Drosophila) -like 1 (Hu antigen R)

other names :

ELAV-like protein 1; ELAV-like protein 1; ELAV-like protein 1; Hu antigen R; hu-antigen R; embryonic lethal, abnormal vision, drosophila, homolog-like 1; ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R); ELAV like RNA binding protein 1; Hu-antigen R; HuR

products gene name :

ELAVL1

other gene names :

ELAVL1; ELAVL1; HUR; Hua; MelG; ELAV1; HUR; HuR

uniprot entry name :

ELAV1_HUMAN

reactivity :

Human

sequence length :

326

specificity :

This assay has high sensitivity and excellent specificity for detection of human ELAVL1. No significant cross-reactivity or interference between human ELAVL1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml-1600 pg/ml. Sensitivity: Typically less than 6.25 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ELAVL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ELAVL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ELAVL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ELAVL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

38201714

ncbi acc num :

NP_001410.2

ncbi gb acc num :

NM_001419.2

uniprot acc num :

Q15717

ncbi mol weight :

36,092 Da

ncbi pathways :

Gene Expression Pathway (105937); Regulation Of MRNA Stability By Proteins That Bind AU-rich Elements Pathway (187211); Stabilization Of MRNA By HuR Pathway (187216)

ncbi summary :

The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy. [provided by RefSeq, Sep 2012]

uniprot summary :

HuR: Involved in 3 -UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5 -UUUU[AG]UUU-3 motif in vitro. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with ANP32A, EIF2C1 and EIF2C2. Ubiquitous. Belongs to the RRM elav family. Protein type: RNA-binding. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol; nucleus. Molecular Function: mRNA binding; protein binding; mRNA 3 -UTR binding; RNA binding; double-stranded RNA binding; nucleotide binding; AU-rich element binding; protein kinase binding. Biological Process: positive regulation of translation; mRNA stabilization; multicellular organismal development; gene expression

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage