Human Rac GTPase-activating protein 1, RACGAP1 ELISA Kit | MyBioSource MBS919252 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Rac GTPase-activating protein 1, RACGAP1 ELISA Kit

catalog :

MBS919252

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS919252

products type :

ELISA Kit

products full name :

Human Rac GTPase-activating protein 1, RACGAP1 ELISA Kit

products short name :

Rac GTPase activating protein 1

products name syn :

Human Rac GTPase-activating protein 1 (RACGAP1) ELISA kit; HsCYK-4; ID-GAP; MgcRacGAP; GTPase activating protein; Rac GTPase activating protein 1

other names :

rac GTPase-activating protein 1; Rac GTPase-activating protein 1; rac GTPase-activating protein 1; protein CYK4 homolg; protein CYK4 homolog; male germ cell RacGap; GTPase activating protein; Rac GTPase activating protein 1; Male germ cell RacGap; MgcRacGAP; Protein CYK4 homolog; CYK4; HsCYK-4

products gene name :

RACGAP1

other gene names :

RACGAP1; RACGAP1; CYK4; ID-GAP; HsCYK-4; MgcRacGAP; KIAA1478; MGCRACGAP; MgcRacGAP; CYK4; HsCYK-4

uniprot entry name :

RGAP1_HUMAN

reactivity :

Human

sequence length :

632

specificity :

This assay has high sensitivity and excellent specificity for detection of human RACGAP1. No significant cross-reactivity or interference between human RACGAP1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml -1600 pg/ml. Sensitivity: The minimum detectable dose of human RACGAP1 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RACGAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RACGAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RACGAP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RACGAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

186910300

ncbi acc num :

NP_001119575.1

ncbi gb acc num :

NM_001126103.1

uniprot acc num :

Q9H0H5

ncbi mol weight :

71,027 Da

ncbi pathways :

Aurora B Signaling Pathway (138080); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Hemostasis Pathway (106028); Kinesins Pathway (187197); RAC1 Signaling Pathway (169355); Regulation Of CDC42 Activity Pathway (137990); Regulation Of RAC1 Activity Pathway (169346); Rho GTPase Cycle Pathway (106503); Signal Transduction Pathway (477114); Signal Transduction Of S1P Receptor Pathway (198819)

ncbi summary :

The protein encoded by this gene belongs to the GTPase-activating protein (GAP) family. GAPs bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, GAPs negatively regulate Rho-mediated signals. This protein plays a regulatory role in initiation of cytokinesis, controlling cell growth and differentiation of hematopoietic cells, regulating spermatogenesis, and in neuronal proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2011]

uniprot summary :

MgcRacGAP: a rac GTPase activating protein. Plays an important role in cytokinesis by regulating cortical movement through RhoA. Phosphorylation by Aurora B apparently induces GAP activity toward RhoA. Protein type: GAPs, Rac/Rho; GAPs. Chromosomal Location of Human Ortholog: 12q13.12. Cellular Component: nucleoplasm; microtubule; extrinsic to internal side of plasma membrane; acrosome; spindle midzone; midbody; nucleus; cytosol; cleavage furrow. Molecular Function: protein binding; gamma-tubulin binding; phosphatidylinositol-3,4,5-triphosphate binding; metal ion binding; microtubule binding; beta-tubulin binding; protein kinase binding; alpha-tubulin binding; GTPase activator activity. Biological Process: positive regulation of cytokinesis; regulation of attachment of spindle microtubules to kinetochore; spindle midzone assembly involved in mitosis; antigen processing and presentation of exogenous peptide antigen via MHC class II; sulfate transport; microtubule-based movement; regulation of small GTPase mediated signal transduction; embryonic development; cytokinesis after mitosis; small GTPase mediated signal transduction; neuroblast proliferation; spermatogenesis; blood coagulation; cytokinesis, contractile ring formation; positive regulation of GTPase activity

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage