Mouse Beta-type platelet-derived growth factor receptor, PDGFRB ELISA Kit | MyBioSource MBS919047 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Beta-type platelet-derived growth factor receptor, PDGFRB ELISA Kit

catalog :

MBS919047

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

citations: 1

Reference
Button E, Boyce G, Wilkinson A, Stukas S, Hayat A, Fan J, et al. ApoA-I deficiency increases cortical amyloid deposition, cerebral amyloid angiopathy, cortical and hippocampal astrogliosis, and amyloid-associated astrocyte reactivity in APP/PS1 mice. Alzheimers Res Ther. 2019;11:44 pubmed publisher

product information

catalog number :

MBS919047

products type :

ELISA Kit

products full name :

Mouse Beta-type platelet-derived growth factor receptor, PDGFRB ELISA Kit

products short name :

platelet-derived growth factor receptor, beta polypeptide

products name syn :

Mouse Beta-type platelet-derived growth factor receptor (PDGFRB) ELISA kit; CD140B; JTK12; PDGFR; PDGFR1; CD140 antigen-like family member B; PDGF-R-beta; platelet-derived growth factor receptor beta; platelet-derived growth factor receptor; beta polypeptide

other names :

platelet-derived growth factor receptor beta isoform 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta; PDGFR-beta; PDGF-R-beta; PDGF beta chain; CD140 antigen-like family member B; platelet-derived growth factor receptor 1; beta platelet-derived growth factor receptor; beta-type platelet-derived growth factor receptor; platelet-derived growth factor receptor beta variant 1; platelet derived growth factor receptor, beta polypeptide; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1; CD_antigen: CD140b

products gene name :

PDGFRB

other gene names :

Pdgfrb; Pdgfrb; Pdgfr; CD140b; PDGFR-1; AI528809; Pdgfr; Pdgfr1; PDGF-R-beta; PDGFR-beta; PDGFR-1

uniprot entry name :

PGFRB_MOUSE

reactivity :

Mouse

sequence length :

1099

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse PDGFRB. No significant cross-reactivity or interference between mouse PDGFRB and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml -1500 pg/ml. Sensitivity: 5.86 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDGFRB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGFRB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFRB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFRB bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

226342982

ncbi acc num :

NP_001139740.1

ncbi gb acc num :

NM_001146268.1

uniprot acc num :

P05622

ncbi mol weight :

122,806 Da

ncbi pathways :

Calcium Signaling Pathway (83247); Calcium Signaling Pathway (459); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Downstream Signal Transduction Pathway (575396); ESC Pluripotency Pathways (198374); Focal Adhesion Pathway (198353); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478); Gap Junction Pathway (83269)

uniprot summary :

PDGFRB: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation, thereby recruiting SH2-containing proteins including Grb2, Src, GAP, PTPN11, PI3 kinase, PLC-gamma and Nck. Regulates cell growth, actin reorganization, migration and differentiation. A variety of myeloproliferative disorders and cancers result from translocations that activate PDGFRbeta by fusion with proteins such as TEL/ETV6, H2, CEV14/TRP11, rabaptin 5, and huntington interacting protein 1. Gleevec treatment of TEL fusions has been successful. Overexpressed in metastatic medulloblastoma. Inhibitors: Gleevec, Sutent. Protein type: Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Oncoprotein; Protein kinase, TK; Kinase, protein; TK group; PDGFR family. Cellular Component: cell surface; focal adhesion; membrane; lysosome; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; cytoplasmic vesicle; nucleus; intrinsic to plasma membrane. Molecular Function: platelet-derived growth factor binding; nucleotide binding; platelet-derived growth factor receptor binding; platelet-derived growth factor receptor activity; transmembrane receptor protein tyrosine kinase activity; protein kinase binding; protein kinase activity; transferase activity; protein binding; signal transducer activity; protein-tyrosine kinase activity; platelet-derived growth factor beta-receptor activity; transferase activity, transferring phosphorus-containing groups; phosphoinositide 3-kinase binding; kinase activity; ATP binding; receptor binding. Biological Process: positive regulation of phosphoprotein phosphatase activity; peptidyl-tyrosine phosphorylation; positive regulation of smooth muscle cell proliferation; multicellular organismal development; protein amino acid autophosphorylation; positive regulation of collagen biosynthetic process; platelet-derived growth factor receptor signaling pathway; cardiac myofibril assembly; chemotaxis; signal transduction; positive regulation of smooth muscle cell migration; protein amino acid phosphorylation; smooth muscle development; positive regulation of MAP kinase activity; nitrogen compound metabolic process; positive regulation of cell proliferation; tissue homeostasis; hemopoiesis; phosphatidylinositol metabolic process; kidney development; regulation of peptidyl-tyrosine phosphorylation; cell migration; phosphoinositide-mediated signaling; positive regulation of mitosis; adrenal gland development; in utero embryonic development; positive regulation of chemotaxis; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; glycosaminoglycan biosynthetic process; skeletal morphogenesis; regulation of actin cytoskeleton organization and biogenesis; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis; positive regulation of cell migration

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage