Human Fibroblast growth factor 14, FGF14 ELISA Kit | MyBioSource MBS918979 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Fibroblast growth factor 14, FGF14 ELISA Kit

catalog :

MBS918979

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS918979

products type :

ELISA Kit

products full name :

Human Fibroblast growth factor 14, FGF14 ELISA Kit

products short name :

fibroblast growth factor 14

products name syn :

Human Fibroblast growth factor 14 (FGF14) ELISA kit; RP11-397O8.6; FHF4; MGC119129; SCA27; bA397O8.2; fibroblast growth factor homologous factor 4; fibroblast growth factor 14

other names :

fibroblast growth factor 14 isoform 1A; Fibroblast growth factor 14; fibroblast growth factor 14; bA397O8.2; fibroblast growth factor homologous factor 4; fibroblast growth factor 14; Fibroblast growth factor homologous factor 4; FHF-4

products gene name :

FGF14

other gene names :

FGF14; FGF14; FHF4; FHF-4; SCA27; FGF-14; FHF4; FGF-14; FHF-4

uniprot entry name :

FGF14_HUMAN

reactivity :

Human

sequence length :

247

specificity :

This assay has high sensitivity and excellent specificity for detection of human FGF14. No significant cross-reactivity or interference between human FGF14 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.5 pg/ml -1500 pg/ml. Sensitivity: The minimum detectable dose of human FGF14 is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF14 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4758368

ncbi acc num :

NP_004106.1

ncbi gb acc num :

NM_004115.3

uniprot acc num :

Q92915

ncbi mol weight :

28,462 Da

ncbi pathways :

MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Melanoma Pathway (83114); Melanoma Pathway (526); Pathways In Cancer (83105); Regulation Of Actin Cytoskeleton Pathway (198874); Regulation Of Actin Cytoskeleton Pathway (83089); Regulation Of Actin Cytoskeleton Pathway (500)

ncbi summary :

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. A mutation in this gene is associated with autosomal dominant cerebral ataxia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

FGF14: Probably involved in nervous system development and function. Defects in FGF14 are the cause of spinocerebellar ataxia type 27 (SCA27). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA27 is an autosomal dominant cerebellar ataxia (ADCA). It is a slowly progressive disorder, with onset in late-childhood to early adulthood, characterized by ataxia with tremor, orofacial dyskinesia, psychiatric symptoms and cognitive deficits. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine; Cell development/differentiation. Chromosomal Location of Human Ortholog: 13q34. Cellular Component: extracellular region; nucleus. Molecular Function: heparin binding; growth factor activity; fibroblast growth factor receptor binding. Biological Process: nervous system development; synaptic transmission; fibroblast growth factor receptor signaling pathway; cell-cell signaling; adult locomotory behavior; neuromuscular process; JNK cascade; signal transduction. Disease: Spinocerebellar Ataxia 27

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage