ELISA/assay

Human Ephrin type-B receptor 1, EPHB1 ELISA Kit

MBS918842

48-Strip-Wells

510 USD

ELISA Kit

Human Ephrin type-B receptor 1, EPHB1 ELISA Kit

EPH receptor B1

Human Ephrin type-B receptor 1 (EPHB1) ELISA kit; ELK; EPHT2; FLJ37986; Hek6; NET; eph tyrosine kinase 2; ephrin receptor EphB1; soluble EPHB1 variant 1; EPH receptor B1

ephrin type-B receptor 1; Ephrin type-B receptor 1; ephrin type-B receptor 1; EK6; EPH-like kinase 6; eph tyrosine kinase 2; soluble EPHB1 variant 1; tyrosine-protein kinase receptor EPH-2; neuronally-expressed EPH-related tyrosine kinase; EPH receptor B1; ELK; EPH tyrosine kinase 2; EPH-like kinase 6; EK6; hEK6; Neuronally-expressed EPH-related tyrosine kinase; NET; Tyrosine-protein kinase receptor EPH-2

EPHB1

EPHB1; EPHB1; ELK; NET; Hek6; EPHT2; ELK; EPHT2; HEK6; NET; EK6; hEK6; NET

EPHB1_HUMAN

Human

984

This assay has high sensitivity and excellent specificity for detection of human EPHB1. No significant cross-reactivity or interference between human EPHB1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of human EPHB1 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EPHB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPHB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPHB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPHB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4758284

NP_004432.1

NM_004441.4

P54762

109,885 Da

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); EPHB Forward Signaling Pathway (138047); Ephrin B Reverse Signaling Pathway (138052); EphrinB-EPHB Pathway (137951)

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008]

EphB1: a receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-B family: ephrin-B1, -B2 and -B3. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. The ligand-activated form of EphB1 interacts with GRB2, GRB10 and NCK through their respective SH2 domains. Four alternatively spliced isoforms are known. Protein type: Membrane protein, integral; Protein kinase, tyrosine (receptor); Protein kinase, TK; EC 2.7.10.1; Kinase, protein; TK group; Eph family. Chromosomal Location of Human Ortholog: 3q21-q23. Cellular Component: early endosome membrane; axon; integral to plasma membrane; dendrite; extracellular region; plasma membrane; cytosol; lipid raft. Molecular Function: protein binding; transmembrane-ephrin receptor activity; axon guidance receptor activity; ATP binding. Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; central nervous system projection neuron axonogenesis; optic nerve morphogenesis; regulation of JNK cascade; cell-substrate adhesion; positive regulation of synaptogenesis; camera-type eye morphogenesis; neurogenesis; establishment of cell polarity; ephrin receptor signaling pathway; angiogenesis; detection of temperature stimulus involved in sensory perception of pain; retinal ganglion cell axon guidance

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800