ELISA/assay

Rat N-acetylaspartate synthetase, NAT8L ELISA Kit

MBS918714

96 Strip Wells

760 USD

ELISA Kit

Rat N-acetylaspartate synthetase, NAT8L ELISA Kit

N-acetyltransferase 8-like (GCN5-related, putative)

Rat N-acetylaspartate synthetase (NAT8L) ELISA kit; CML3; FLJ37478; MGC117272; NAT8-LIKE; ; N-acetyltransferase 8-like (GCN5-related; putative)

N-acetylaspartate synthetase; N-acetylaspartate synthetase; N-acetylaspartate synthetase; NAA synthetase; N-acetyltransferase 8-like protein; N-acetyltransferase 8-like; N-acetyltransferase 8-like protein (EC:2.3.1.17)

NAT8L

Nat8l; Nat8l; RGD1305719; NAA synthetase

NAT8L_RAT

Rat

299

This assay has high sensitivity and excellent specificity for detection of rat NAT8L. No significant cross-reactivity or interference between rat NAT8L and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of rat N-acetylaspartate synthetase (NAT8L) concentrations in serum, plasma, tissue homogenates, cell lysates. Detection Range: 31.25 pg/ml -2000 pg/ml.

Sensitivity: The minimum detectable dose of rat NAT8L is typically less than 7.81 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NAT8L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAT8L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAT8L is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAT8L bound in the initial step. The color development is stopped and the intensity of the color is measured.

300794296

NP_001178610.1

NM_001191681.1

D3ZVU9

32,719 Da

Function: Plays a role in the regulation of lipogenesis by producing N-acetylaspartate acid (NAA), a brain-specific metabolite. NAA occurs in high concentration in brain and its hydrolysis plays a significant part in the maintenance of intact white matter. Promotes dopamine uptake by regulating TNF-alpha expression. Attenuates methamphetamine-induced inhibition of dopamine uptake . Catalytic activity: Acetyl-CoA + L-aspartate = CoA + N-acetyl-L-aspartate. Enzyme regulation: Aminooxyacetic acid (AOAA) blocks its activity in both cytoplasm and mitochondria

96 Strip Wells

760 USD