Human Eosinophil peroxidase, EPX ELISA Kit | MyBioSource MBS918517 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Eosinophil peroxidase, EPX ELISA Kit

catalog :

MBS918517

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS918517

products type :

ELISA Kit

products full name :

Human Eosinophil peroxidase, EPX ELISA Kit

products short name :

eosinophil peroxidase

products name syn :

Human Eosinophil peroxidase (EPX) ELISA kit; EPO; EPP; EPX-PEN; ; eosinophil peroxidase

other names :

eosinophil peroxidase preproprotein; Eosinophil peroxidase; eosinophil peroxidase; eosinophil peroxidase

products gene name :

EPX

other gene names :

EPX; EPX; EPO; EPP; EPX-PEN; EPER; EPO; EPP; EPO

uniprot entry name :

PERE_HUMAN

reactivity :

Human

sequence length :

715

specificity :

This assay has high sensitivity and excellent specificity for detection of human EPX. No significant cross-reactivity or interference between human EPX and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: The minimum detectable dose of human EPX is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EPX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPX is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPX bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4503595

ncbi acc num :

NP_000493.1

ncbi gb acc num :

NM_000502.4

uniprot acc num :

P11678

ncbi mol weight :

81,040 Da

ncbi pathways :

Asthma Pathway (83120); Asthma Pathway (532)

ncbi summary :

This gene is a member of the peroxidase gene family and is expressed in eosinophils. The encoded precursor protein is processed into covalently attached heavy and light chains to form the mature enzyme, which functions as an oxidant. The enzyme is released at sites of parasitic infection or allergen stimulation to mediate lysis of protozoa or parasitic worms. The gene is found in a cluster of three peroxidase genes at chromosome 17q23. Mutations in this gene result in eosinophil peroxidase deficiency. [provided by RefSeq, Sep 2009]

uniprot summary :

EPX: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. Defects in EPX are the cause of eosinophil peroxidase deficiency (EPD). EPD is an autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining. Belongs to the peroxidase family. XPO subfamily. Protein type: Oxidoreductase; EC 1.11.1.7. Chromosomal Location of Human Ortholog: 17q23.1. Molecular Function: peroxidase activity; metal ion binding; heme binding. Biological Process: positive regulation of interleukin-4 production; negative regulation of interleukin-10 production; hydrogen peroxide catabolic process; negative regulation of interleukin-5 production; response to oxidative stress; defense response to nematode. Disease: Eosinophil Peroxidase Deficiency

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage