catalog number :
MBS918432
products type :
ELISA Kit
products full name :
Pig Colipase, CLPS ELISA Kit
products short name :
colipase, pancreatic
products name syn :
Pig Colipase (CLPS) ELISA kit; colipase; pancreatic colipase preproprotein; colipase; pancreatic
other names :
colipase; Colipase; colipase; procolipase II; pancreatic colipase; Procolipase II
products gene name :
CLPS
other gene names :
CLPS; CLPS
uniprot entry name :
COL_PIG
specificity :
This assay has high sensitivity and excellent specificity for detection of Pig CLPS. No significant cross-reactivity or interference between Pig CLPS and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 mU/ml-1000 mU/ml. Sensitivity: 3.9 mU/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Digestion
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CLPS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CLPS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLPS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLPS bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_999368.1
ncbi gb acc num :
NM_214203.1
ncbi mol weight :
12,140 Da
ncbi pathways :
Digestion Of Dietary Lipid Pathway (578070); Fat Digestion And Absorption Pathway (194378); Fat Digestion And Absorption Pathway (194324); Lipid Digestion, Mobilization, And Transport Pathway (578069); Metabolism Pathway (578053); Metabolism Of Lipids And Lipoproteins Pathway (578068)
uniprot summary :
CLPS: Colipase is a cofactor of pancreatic lipase. It allows the lipase to anchor itself to the lipid-water interface. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase. Belongs to the colipase family. Protein type: Secreted; Secreted, signal peptide; Activator. Molecular Function: enzyme activator activity. Biological Process: positive regulation of catalytic activity; digestion; lipid catabolic process
size4 :
10x96-Strip-Wells