Human Complement C1q subcomponent subunit A, C1QA ELISA Kit | MyBioSource MBS918393 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Complement C1q subcomponent subunit A, C1QA ELISA Kit

catalog :

MBS918393

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS918393

products type :

ELISA Kit

products full name :

Human Complement C1q subcomponent subunit A, C1QA ELISA Kit

products short name :

complement component 1, q subcomponent, A chain

products name syn :

Human Complement C1q subcomponent subunit A (C1QA) ELISA kit; OTTHUMP00000197085; complement component 1; q subcomponent; alpha polypeptide; complement component C1q; A chain; complement component 1; q subcomponent; A chain

other names :

complement C1q subcomponent subunit A; Complement C1q subcomponent subunit A; complement C1q subcomponent subunit A; complement component C1q, A chain; complement component 1, q subcomponent, alpha polypeptide; complement component 1, q subcomponent, A chain

products gene name :

C1QA

other gene names :

C1QA; C1QA

uniprot entry name :

C1QA_HUMAN

reactivity :

Human

sequence length :

245

specificity :

This assay has high sensitivity and excellent specificity for detection of Human C1QA. No significant cross-reactivity or interference between Human C1QA and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 9.37 ng/ml-600 ng/ml. Sensitivity: 2.34 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Complement. Protein Biological Process 3: Complement pathway

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C1QA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C1QA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C1QA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C1QA bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

7705753

ncbi acc num :

NP_057075.1

ncbi gb acc num :

NM_015991.2

uniprot acc num :

P02745

ncbi mol weight :

26,017 Da

ncbi pathways :

Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Classical Antibody-mediated Complement Activation Pathway (106409); Complement Activation, Classical Pathway (198823); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Creation Of C4 And C2 Activators Pathway (106407); Immune System Pathway (106386)

ncbi summary :

This gene encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the A-chain polypeptide of human complement subcomponent C1q. [provided by RefSeq, Jul 2008]

uniprot summary :

C1QA iso2: C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2 )-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1p36.12. Cellular Component: collagen; extracellular region; complement component C1 complex. Molecular Function: protein binding. Biological Process: cell-cell signaling; innate immune response; complement activation, classical pathway; complement activation. Disease: C1q Deficiency

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage