catalog number :
MBS918191
products type :
ELISA Kit
products full name :
Human Afamin, AFM ELISA Kit
products short name :
afamin
products name syn :
Human Afamin (AFM) ELISA kit; ALB2; ALBA; ALF; MGC125338; MGC125339; alpha-albumin; afamin
other names :
afamin; Afamin; afamin; alpha-Alb; alpha-albumin; afamin; Alpha-albumin; Alpha-Alb
other gene names :
AFM; AFM; ALF; ALB2; ALBA; ALB2; ALBA; Alpha-Alb
uniprot entry name :
AFAM_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human AFM. No significant cross-reactivity or interference between human AFM and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.9 ng/ml-250 ng/ml. Sensitivity: The minimum detectable dose of human AFM is typically less than 1 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AFM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AFM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFM bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001124.1
ncbi gb acc num :
NM_001133.2
ncbi mol weight :
69,069 Da
ncbi summary :
This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. [provided by RefSeq, Jul 2008]
uniprot summary :
A-albumin: Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier. Belongs to the ALB/AFP/VDB family. Protein type: Secreted, signal peptide; Secreted; Carrier. Chromosomal Location of Human Ortholog: 4q13.3. Cellular Component: extracellular space; extracellular region. Molecular Function: vitamin E binding. Biological Process: vitamin transport
size4 :
10x96-Strip-Wells