product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Gastric triacylglycerol lipase, LIPF ELISA Kit
catalog :
MBS918048
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS918048
products type :
ELISA Kit
products full name :
Mouse Gastric triacylglycerol lipase, LIPF ELISA Kit
products short name :
lipase, gastric
products name syn :
Mouse Gastric triacylglycerol lipase (LIPF) ELISA kit; GL; HGL; HLAL; MGC138477; MGC142271; gastric triacylglycerol lipase; lipase F; lipase; gastric
other names :
gastric triacylglycerol lipase; Gastric triacylglycerol lipase; gastric triacylglycerol lipase; GL; gastric lipase; lipase, gastric
products gene name :
LIPF
other gene names :
Lipf; Lipf; AV082900; 2310051B21Rik; GL; Gastric lipase
uniprot entry name :
LIPG_MOUSE
reactivity :
Mouse
sequence length :
395
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse LIPF. No significant cross-reactivity or interference between mouse LIPF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: The minimum detectable dose of mouse LIPF is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LIPF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LIPF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LIPF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIPF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
13385836
ncbi acc num :
NP_080610.1
ncbi gb acc num :
NM_026334.3
uniprot acc num :
Q9CPP7
ncbi mol weight :
44,637 Da
ncbi pathways :
Acylglycerol Degradation Pathway (421788); Acylglycerol Degradation Pathway (468272); Fat Digestion And Absorption Pathway (194386); Fat Digestion And Absorption Pathway (194324); Fatty Acid Beta Oxidation Pathway (198349); Glycerolipid Metabolism Pathway (83185); Glycerolipid Metabolism Pathway (361); Triacylglyceride Synthesis Pathway (198394); Triacylglycerol Degradation Pathway (143022)
uniprot summary :
LIPF: gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]. Protein type: EC 3.1.1.3; Secreted; Lipid Metabolism - glycerolipid; Secreted, signal peptide; Hydrolase. Cellular Component: extracellular region. Molecular Function: triacylglycerol lipase activity; hydrolase activity; hydrolase activity, acting on ester bonds. Biological Process: lipid metabolic process; lipid catabolic process
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

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