Rat 78 kDa glucose-regulated protein, HSPA5 ELISA Kit | MyBioSource MBS917799 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat 78 kDa glucose-regulated protein, HSPA5 ELISA Kit

catalog :

MBS917799

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS917799

products type :

ELISA Kit

products full name :

Rat 78 kDa glucose-regulated protein, HSPA5 ELISA Kit

products short name :

heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

products name syn :

Rat 78 kDa glucose-regulated protein (HSPA5) ELISA kit; BIP; FLJ26106; GRP78; MIF2; Heat-shock 70kD protein-5 (glucose-regulated protein; 78kD) ; heat shock 70kD protein 5 (glucose-regulated protein; 78kD) ; heat shock 70kDa protein 5; heat shock 70kDa protein 5 (glucose-regulated protein; 78kDa)

other names :

78 kDa glucose-regulated protein; 78 kDa glucose-regulated protein; 78 kDa glucose-regulated protein; GRP 78; GRP-78; heat shock 70kD protein 5; heat shock 70 kDa protein 5; steroidogenesis-activator polypeptide; immunoglobulin heavy chain-binding protein; heat shock 70kDa protein 5 (glucose-regulated protein); heat shock protein 5; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP; Steroidogenesis-activator polypeptide

products gene name :

HSPA5

other gene names :

Hspa5; Hspa5; BIP; GRP78; Grp78; GRP-78; BiP

uniprot entry name :

GRP78_RAT

reactivity :

Rat

sequence length :

654

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat HSPA5. No significant cross-reactivity or interference between Rat HSPA5 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 43.75 pg/ml-2800 pg/ml. Sensitivity: 10.93 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSPA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSPA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSPA5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSPA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

25742763

ncbi acc num :

NP_037215.1

ncbi gb acc num :

NM_013083.2

uniprot acc num :

P06761

ncbi mol weight :

72,347 Da

ncbi pathways :

Activation Of Chaperones By ATF6-alpha Pathway (573658); Activation Of Chaperones By IRE1alpha Pathway (573659); Antigen Processing And Presentation Pathway (83466); Antigen Processing And Presentation Pathway (485); Diabetes Pathways (573655); Disease Pathway (573628); Hemostasis Pathway (573899); MAPK Signaling Pathway (198496); PERK Regulated Gene Expression Pathway (573660); Platelet Activation, Signaling And Aggregation Pathway (573909)

ncbi summary :

secreted protein of the endoplasmic reticulum; may be involved in the assembly of secreted and membrane-bound proteins [RGD, Feb 2006]

uniprot summary :

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Protein type: Heat shock protein; Chaperone. Cellular Component: signalosome; endoplasmic reticulum membrane; smooth endoplasmic reticulum; cell surface; focal adhesion; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen; ER-Golgi intermediate compartment; membrane; melanosome; plasma membrane; integral to endoplasmic reticulum membrane; midbody; nucleus; myelin sheath. Molecular Function: protein domain specific binding; protein binding; enzyme binding; ubiquitin protein ligase binding; unfolded protein binding; ribosome binding; misfolded protein binding; glycoprotein binding; ATP binding. Biological Process: cellular response to glucose starvation; substantia nigra development; cerebellum structural organization; unfolded protein response, activation of signaling protein activity; proteolysis involved in cellular protein catabolic process; positive regulation of protein ubiquitination; cerebellar Purkinje cell layer development; negative regulation of transforming growth factor beta receptor signaling pathway; ER overload response; negative regulation of apoptosis; positive regulation of cell migration

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage