ELISA/assay

Human Liver-expressed antimicrobial peptide 2, LEAP2 ELISA Kit

MBS917663

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human Liver-expressed antimicrobial peptide 2, LEAP2 ELISA Kit

[liver expressed antimicrobial peptide 2]

[Human Liver-expressed antimicrobial peptide 2 (LEAP2) ELISA kit; LEAP-2; liver-expressed antimicrobial peptide 2; liver expressed antimicrobial peptide 2]

[liver-expressed antimicrobial peptide 2; Liver-expressed antimicrobial peptide 2; liver-expressed antimicrobial peptide 2; liver expressed antimicrobial peptide 2]

[LEAP2]

[LEAP2; LEAP2; LEAP-2; LEAP-2]

LEAP2_HUMAN

Human

77

This assay has high sensitivity and excellent specificity for detection of human LEAP2. No significant cross-reactivity or interference between human LEAP2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of human LEAP2 is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LEAP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LEAP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LEAP2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LEAP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

16418465

NP_443203.1

NM_052971.2

Q969E1

8,814 Da

This gene encodes a cysteine-rich cationic antimicrobial peptide that is expressed predominantly in the liver. The mature peptide has activity against gram-positive bacteria and yeasts. [provided by RefSeq, Sep 2014]

LEAP2: Has an antimicrobial activity. Belongs to the LEAP2 family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: extracellular region. Biological Process: defense response to bacterium

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145