catalog number :
MBS917501
products type :
ELISA Kit
products full name :
Mouse Syndecan-3, SDC3 ELISA Kit
products short name :
syndecan 3
products name syn :
Mouse Syndecan-3 (SDC3) ELISA kit; SDCN; SYND3; N-syndecan; syndecan neural type; syndecan proteoglycan 3; syndecan 3
other names :
syndecan-3; Syndecan-3; syndecan-3; syndecan 3
products gene name :
SDC3
other gene names :
Sdc3; Sdc3; Synd3; syn-3; mKIAA0468; Kiaa0468; SYND3
uniprot entry name :
SDC3_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse SDC3. No significant cross-reactivity or interference between mouse SDC3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of mouse SDC3 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SDC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SDC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDC3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDC3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_035650.2
ncbi gb acc num :
NM_011520.3
ncbi mol weight :
46,002 Da
ncbi pathways :
Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); ECM-receptor Interaction Pathway (83265); ECM-receptor Interaction Pathway (479); Malaria Pathway (152666); Malaria Pathway (152657)
uniprot summary :
syndecan-3: Cell surface proteoglycan that may bear heparan sulfate. May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Belongs to the syndecan proteoglycan family. Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis; Receptor, misc. Cellular Component: neuron projection; protein complex; membrane; axon; integral to membrane. Biological Process: cell adhesion
size4 :
10x96-Strip-Wells