Human Microtubule-associated proteins 1A/1B light chain 3B, MAP1LC3B ELISA Kit | MyBioSource MBS917498 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Microtubule-associated proteins 1A/1B light chain 3B, MAP1LC3B ELISA Kit

catalog :

MBS917498

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS917498

products type :

ELISA Kit

products full name :

Human Microtubule-associated proteins 1A/1B light chain 3B, MAP1LC3B ELISA Kit

products short name :

microtubule-associated protein 1 light chain 3 beta

products name syn :

Human Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) ELISA kit; LC3B; MAP1A/1BLC3; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated protein 1 light chain 3 beta

other names :

microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated proteins 1A/1B light chain 3B; microtubule-associated proteins 1A/1B light chain 3B; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1 light chain 3-like protein 2; autophagy-related ubiquitin-like modifier LC3 B; microtubule-associated protein 1 light chain 3 beta; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta

products gene name :

MAP1LC3B

other gene names :

MAP1LC3B; MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; MAP1ALC3; MAP1A/MAP1B LC3 B

uniprot entry name :

MLP3B_HUMAN

reactivity :

Human

sequence length :

125

specificity :

This assay has high sensitivity and excellent specificity for detection of human MAP1LC3B. No significant cross-reactivity or interference between human MAP1LC3B and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 4.68 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MAP1LC3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAP1LC3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP1LC3B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP1LC3B bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

12383056

ncbi acc num :

NP_073729.1

ncbi gb acc num :

NM_022818.4

uniprot acc num :

Q9GZQ8

ncbi mol weight :

14,688 Da

ncbi pathways :

Senescence And Autophagy Pathway (198780)

ncbi summary :

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]

uniprot summary :

LC3B: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Recruits proteins such as SQSTM1 and NBR1 to the autophagosome. Protein type: Autophagy; Microtubule-binding; Vesicle; Ubiquitin-like modifier. Chromosomal Location of Human Ortholog: 16q24.2. Cellular Component: microtubule; extrinsic to membrane; mitochondrion; endomembrane system; autophagic vacuole; intracellular; cytoplasmic vesicle; axoneme; cytosol. Molecular Function: protein binding; microtubule binding; GABA receptor binding. Biological Process: mitochondrion degradation; cellular response to nitrogen starvation; autophagic vacuole formation

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage