catalog number :
MBS917466
products type :
ELISA Kit
products full name :
Mouse 14-3-3 protein zeta/delta, YWHAZ ELISA Kit
products short name :
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
products name syn :
Mouse 14-3-3 protein zeta/delta (YWHAZ) ELISA kit; 14-3-3-zeta; KCIP-1; MGC111427; MGC126532; MGC138156; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 zeta; OTTHUMP00000165851; OTTHUMP00000165858; phospholipase A2; protein kinase C inhibitor protein-; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; zeta polypeptide
other names :
14-3-3 protein zeta/delta isoform 1; 14-3-3 protein zeta/delta; 14-3-3 protein zeta/delta; SEZ-2; KCIP-1; 14-3-3 zeta; protein kinase C inhibitor protein 1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; Protein kinase C inhibitor protein 1; KCIP-1; SEZ-2
products gene name :
YWHAZ
other gene names :
Ywhaz; Ywhaz; AI596267; AL022924; AU020854; 14-3-3zeta; 1110013I11Rik; KCIP-1
uniprot entry name :
1433Z_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse YWHAZ. No significant cross-reactivity or interference between Mouse YWHAZ and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Assay Type: Sandwich
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for YWHAZ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YWHAZ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAZ is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAZ bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001240734.1
ncbi gb acc num :
NM_001253805.1
ncbi mol weight :
27,771 Da
ncbi pathways :
Alpha6-Beta4 Integrin Signaling Pathway (198373); Calcium Regulation In The Cardiac Cell Pathway (198327); Cell Cycle Pathway (83251); Cell Cycle Pathway (463); Epstein-Barr Virus Infection Pathway (585576); Epstein-Barr Virus Infection Pathway (587115); GP1b-IX-V Activation Signalling Pathway (575490); Hemostasis Pathway (575479); IL-3 Signaling Pathway (198420); IL-5 Signaling Pathway (198281)
uniprot summary :
14-3-3 zeta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Phosphorylation apparently disrupts homodimerization. Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold. Cellular Component: extracellular space; mast cell granule; focal adhesion; protein complex; mitochondrion; perinuclear region of cytoplasm; postsynaptic density; cytoplasm; leading edge; nucleus. Molecular Function: protein domain specific binding; identical protein binding; protein binding; protein complex binding; transcription factor binding; protein kinase binding. Biological Process: response to drug; histamine secretion by mast cell; establishment of Golgi localization; protein targeting to mitochondrion; protein targeting
size4 :
10x96-Strip-Wells